Recombinant Human BAFF Protein
|B cell activating factor||
Recombinant Human B cell activating factor (BAFF), is a Type II member of the TNF superfamily. Although it is typically a transmembrane protein expressed on T cells, macrophages and dendritic cells, it can also be cleaved in the extracellular region to produce a soluble form detectable in the serum. BAFF is thought to be important for the survival and maturation of peripheral B cells. This recombinant human BAFF contains the TNF-like domain.
Determined by a mouse splenocyte survival assay. The expected ED50 for this effect is 0.5-3.0 µg/ml.
|153 αα||17.2 kDa||Q9Y275
Sterile filtered white lyophilized (freeze-dried) powder.
Lyophilized from 10 mM NaP, pH 7.5.
It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Protein Content determined by
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
BAFF, Tumor necrosis factor ligand superfamily member 13B
MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL
RESEARCH USE ONLY