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Recombinant Human BAFF Protein

   
B cell activating factor  

Recombinant Human B cell activating factor (BAFF), is a Type II member of the TNF superfamily. Although it is typically a transmembrane protein expressed on T cells, macrophages and dendritic cells, it can also be cleaved in the extracellular region to produce a soluble form detectable in the serum. BAFF is thought to be important for the survival and maturation of peripheral B cells. This recombinant human BAFF contains the TNF-like domain.

 

Determined by a mouse splenocyte survival assay. The expected ED50 for this effect is 0.5-3.0 µg/ml.

         
153 αα  17.2 kDa Q9Y275
>90% E. coli -20oC


Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from 10 mM NaP, pH 7.5.

Reconstitution

It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Alternative Names

BAFF, Tumor necrosis factor ligand superfamily member 13B

AA Sequence

MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL

 

RESEARCH USE ONLY