Recombinant Human BAFF protein
Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required.
Alias
Tumor necrosis factor ligand superfamily member 13B
Description:
Recombinant Human B cell activating factor (BAFF), is a Type II member of the TNF superfamily. Although it is typically a transmembrane protein expressed on T cells, macrophages and dendritic cells, it can also be cleaved in the extracellular region to produce a soluble form detectable in the serum. BAFF is thought to be important for the survival and maturation of peripheral B cells. This recombinant human BAFF is produced in E.coli and is a 153 amino acid polypeptide which contains the TNF-like domain and has a molecular mass of 17,171 Da
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Source:
E.coli
Formulation:
Lyophilized from 10 mM NaP, pH 7.5.
Reconstitution:
It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity
Greater than 90% as determined by:
Analysis by RP-HPLC.
Reducing and non-reducing SDS-PAGE.
Protein Content determined by:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Biological Activity:
Determined by a mouse splenocyte survival assay. The expected ED50 for this effect is 0.5-3.0 µg/ml.
AA Sequence:
MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL
RESEARCH USE ONLY
