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Recombinant Human IFN-gamma Protein

   
Interferon-gamma  

Interferon-gamma (IFN-gamma) is a major immune-modulating molecule produced mainly by T cells and natural killer cells activated by antigens, mitogens or alloantigens. Most immune cells express IFN-gamma receptors and respond to IFN-gamma induced signaling by up-regulating MHC class I expression. Human IFN-gamma does not show cross-reactivity with mouse. Recombinant human IFN-gamma is a non-glycosylated protein.

 

The activity is measured in antiviral assays using virally infected L929 cells and is typically less than 0.1 ng/ml. This corresponds to an expected specific activity of 1 x 107 units/mg.

         
144 αα  16.9 kDa P01579
>95% E. coli -20°C

 

Product Citation

Reduced PMA enhances the responsiveness of transfected THP-1 macrophages to polarizing stimuli.

Marten, B., et al. 2014. Journal of Immunology Methods.


Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a sterile filtered solution containing 5 mM Na3PO4 and 50 mM NaCl, pH 7.2.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration ranging from 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Alternative Names

Interferon gamma, immune interferon, type II interferon, T cell interferon, IFG, IFI, MAF

AA Sequence

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

 

RESEARCH USE ONLY