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Recombinant Human IFN-gamma Protein

   
Interferon-Gamma  

The major interferon is produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. The synthesis of IFN-gamma is induced, among other things, by Interleukin-2, FGF-basic, and EGF. Recombinant Human Interferon gamma is a single, non-glycosylated polypeptide chain.

 

Recombinant Interferon gamma is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay is less then 0.05 ng/ml.

         
144 αα  16.9 kDa P01579
>95% E. coli -20oC

 

Product Citation

Reduced PMA enhances the responsiveness of transfected THP-1 macrophages to polarizing stimuli.

Marten, B., et al. 2014. Journal of Immunology Methods.


Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Human IFN-gamma was lyophilized from 5 mM NaP, pH 7.2 + 50 mM NaCl.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration ranging from 0.1mg/ml, which can be further diluted into other aqueous solutions. Spin out slight precipitate, and use.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Alternative Names

interferon gamma, Immune interferon, IFN-gamma, IFG, IFI

AA Sequence

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

 

RESEARCH USE ONLY