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Recombinant Mouse FGF-1 Protein

   
Fibroblast Growth Factor 1   Recombinant Mouse Fibroblast Growth Factor-acidic is one of over 20 members of the FGF family. The fibroblast growth factors play a central role in prenatal development and growth and regeneration of cells during the proliferation and differentiation stages. M -FGF-acidic is non-glycosylated heparin binding protein.    Recombinant Mouse FGF-alpha is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is 0.04ng/ml.
         
141 αα  15.9 kDa NP_034327
>98% E. coli -20oC


Physical Appearance

Sterile Filtered white lyophilized (freeze- dried) powder.

Formulation

Lyophilized at a concentration of 1 mg/ml in 5 mM NaP, 7.5 + 50 mM NaCl.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 – 0.5 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Alternative Names

fibroblast growth factor 1 (acidic),  GLIO703, ECGF-beta,   Acidic fibroblast growth factor, FGFA,   Beta-endothelial cell growth factor, ECGFA,  HBGF-1, HBGF,   heparin-binding growth factor 1, ECGF,  endothelial cell growth factor - beta, AFGF,     endothelial cell growth factor - alpha, ECGFB,     OTTHUMP00000199419, FGF-alpha, aFGF

AA Sequence

MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

RESEARCH USE ONLY