recombinant mouse FGF1 protein
Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required.
Aliases
fibroblast growth factor 1 (acidic), GLIO703, ECGF-beta, Acidic fibroblast growth factor, FGFA, Beta-endothelial cell growth factor, ECGFA, HBGF-1, HBGF, heparin-binding growth factor 1, ECGF, endothelial cell growth factor - beta, AFGF, endothelial cell growth factor - alpha, ECGFB, OTTHUMP00000199419, FGF-alpha, aFGF
Description:
Recombinant Mouse Fibroblast Growth Factor-acidic is one of over 20 members of the FGF family. The fibroblast growth factors play a central role in prenatal development and growth and regeneration of cells during the proliferation and differentiation stages. M -FGF-acidic is non-glycosylated heparin binding protein comprised of 141 amino acid residues with a molecular weight of 15.9 kDa.
Physical Appearance:
Sterile Filtered white lyophilized (freeze- dried) powder.
Source:
E.coli
Formulation:
Lyophilized at a concentration of 1 mg/ml in 5 mM NaP, 7.5 + 50 mM NaCl.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 – 0.5 mg’s/ml, which can be further diluted into other aqueous solutions.
Stability:
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity Greater than 98% as determined by:
Analysis by RP- HPLC.
Reducing and non-reducing SDS- PAGE
Endotoxin Level:
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Protein Content determined by:
UV spectroscopy at 280 nm.
Analysis by RP-HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Biological Activity:
Recombinant Mouse FGF-alpha is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is 0.04ng/ml.
AA Sequence:
MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
