recombinant mouse IGF1 protein

Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required. 

Aliases

insulin-like growth factor 1 (somatomedin C), insulin-like growth factor 1, IGF1A1,      IGF-IB Mechano growth factor, IGF-I,  insulin-like growth factor I, MGF2,  insulin-like growth factor IB,  insulin-like growth factor IA IGF-IA ,  IBP1, somatomedin-C, Somatomedin-C IGFI

Description:

A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor. Recombinant mouse IGF-1 produced in E.Coli is a single, non -glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7600 Dalton.

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Physical Appearance:

Sterile Filtered white lyophilized (freeze-dried) powder.

Source:

E.coli

Formulation:

Recombinant Mouse IGF-1 was lyophilized with no additives.

Reconstitution:

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg’s/ml, which can be further diluted into other aqueous solutions.

Stability:

Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Purity Greater than 98% as determined by:

Analysis by RP-HPLC.

Reducing and non-reducing SDS- PAGE.

Endotoxin Level:

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Protein Content determined by:

UV spectroscopy at 280 nm.

Analysis by RP-HPLC calibrated against a known standard.

Quantitation on SDS-PAGE against a known standard.

Biological Activity:

Recombinant Mouse IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of MCF -7 cells, is less than 1.0 ng/ml.

AA Sequence:

GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA