Media & Matrices Exosomes Matrigen Softwell Growth Factors Small Molecules Gene Knock-Up Karyotype Service

Recombinant Mouse IGF-1 Protein

   

Insulin Like Growth Factor 1

 

A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor. Recombinant mouse IGF-1 is a single, non-glycosylated, polypeptide chain. 

 

Recombinant Mouse IGF-1 is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of MCF -7 cells, is less than 1.0 ng/ml.

         
70 αα  7.6 kDa Q8CAR0
>98% E. coli -20oC


Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Recombinant Mouse IGF-1 was lyophilized with no additives.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Endotoxin Level

Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Alternative Names

insulin-like growth factor 1 (somatomedin C), insulin-like growth factor 1, IGF1A1, IGF-IB Mechano growth factor, IGF-I, insulin-like growth factor I, MGF2, insulin-like growth factor IB, insulin-like growth factor IA, IGF-IA, IBP1, somatomedin-C, Somatomedin-C IGFI

AA Sequence

GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA

 

RESEARCH USE ONLY