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Recombinant Mouse IL-9 Protein

   
Interleukin 9   Interleukin (IL)-9 is known to regulate many cell types involved in T-helper type 2 responses classically associated with asthma, including B- and T- lymphocytes, mast cells, eosinophils and epithelial cells. Growing evidence obtained from human genomic analysis and antigen-challenged transgenic mice suggests that interleukin-9 (IL-9) is a candidate factor in immunoglobulin E (IgE) production and thus is thought to be associated with bronchial inflammation and bronchial hyperresponsiveness (BHR). Recombinant Mouse IL-9 is a non-glycosylated, single polypeptide chain.    Recombinant mouse IL-9 is fully bioactive when compared to a standard. Mouse IL-9 induces a dose-dependent proliferation of mouse Mo7e cells with an ED50 of 0.382 ng/ml.
         
127 αα 14.3 kDa P15247
>96% E. coli -20oC


Physical Appearance

Sterile Filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from 10mM Sodium Phosphate, pH 7.5

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Endotoxin Level

Endotoxin level is <0.01ng/ug or <0.1EU/ug.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP- HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Alternative Names

interleukin 9, Cytokine P40, IL-9,  interleukin-9, P40,  p40 cytokine, HP40, homolog of mouse T cell and mast cell growth factor 40, T-cell growth factor P40, p40 T-cell and mast cell growth factor 

AA Sequence

MQRCSTTWGI RDTNYLIENL KDDPPSKCSC SGNVTSCLCL SVPTDDCTTP CYREGLLQLT NATQKSRLLP VFHRVKRIVE VLKNITCPSF SCEKPCNQTM AGNTMSFLKS LLGTFQKTEM QRQKSRP

RESEARCH USE ONLY