recombinant mouse IL9 protein
Growth factors from Cell Guidance Systems are high purity suitable for the most demanding applications where biological activity is required.
Aliases
interleukin 9, Cytokine P40, IL-9, interleukin-9, P40, p40 cytokine, HP40, homolog of mouse T cell and mast cell growth factor 40, T-cell growth factor P40, p40 T-cell and mast cell growth factor
Description:
Interleukin (IL)-9 is known to regulate many cell types involved in T- helper type 2 responses classically associated with asthma, including B- and T- lymphocytes, mast cells, eosinophils and epithelial cells. Growing evidence obtained from human genomic analysis and antigen-challenged transgenic mice suggests that interleukin-9 (IL-9) is a candidate factor in immunoglobulin E (IgE) production and thus is thought to be associated with bronchial inflammation and bronchial hyperresponsiveness (BHR). Recombinant Mouse IL-9 produced in E.Coli is a non-glycosylated, single polypeptide chain containing 127amino acids and having a molecular mass of 14,298 Daltons.
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Source:
E.coli
Formulation:
Lyophilized from 10mM Sodium Phosphate, pH 7.5
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability:
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Purity Greater than 96% as determined by:
Analysis by RP-HPLC.
Reducing and non-reducing SDS-PAGE.
Endotoxin Level:
Endotoxin level is <0.01ng/ug or <0.1EU/ug.
Protein Content determined by:
UV spectroscopy at 280 nm.
Analysis by RP- HPLC calibrated against a known standard.
Quantitation on SDS-PAGE against a known standard.
Biological Activity:
Recombinant mouse IL-9 is fully bioactive when compared to a standard. Mouse IL-9 induces a dose-dependent proliferation of mouse Mo7e cells with an ED50 of 0.382 ng/ml.
AA Sequence:
MQRCSTTWGI RDTNYLIENL KDDPPSKCSC SGNVTSCLCL SVPTDDCTTP CYREGLLQLT NATQKSRLLP VFHRVKRIVE VLKNITCPSF SCEKPCNQTM AGNTMSFLKS LLGTFQKTEM QRQKSRP
RESEARCH USE ONLY
