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Recombinant Human MCSF Protein

   
Macrophage Colony Stimulating Factor   Macrophage colony stimulating factor (M-CSF) is one of the glycoproteins called colony-stimulating factors (CSFs).A mononuclear phagocyte colony- stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo. Recombinant Human M-CSF is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8 KDa.  

Recombinant Human Macrophage Colony Stimulating Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of mouse NFS-60 cells is typcially less than 2.0 ng/mL.

         
159 αα 36.8 kDa P09603
>97% E. coli -20oC


Physical Appearance

Sterile Filtered white lyophilized (freeze-dried) powder.

Formulation

Human MCSF is lyophilized in 10 mM NaP, 8.0 + 50 mM NaCl, at a concentration of 1 mg/ml.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Endotoxin Level

Endotoxin level is <0.01ng/ug or <0.1EU/ug.

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP- HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Alternative Names

Macrophage colony stimulating factor (M-CSF)

AA Sequence

MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS

RESEARCH USE ONLY