Recombinant Human MCSF

Code Description Price Qty
GFH3-2 Recombinant Human MCSF, 2 μg £44.21
GFH3-10 Recombinant Human MCSF, 10 μg £105.00
GFH3-100 Recombinant Human MCSF, 100 μg £339.50
GFH3-500 Recombinant Human MCSF, 500 μg £1,600.00
GFH3-1000 Recombinant Human MCSF, 1 mg £2,871.79
Induced proliferation of NFS-60 cells assay for Human MCSF. Cell proliferation was measured to calculate the ED50.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human MCSF has a predicted Mw of 37.1 kDa.

Description

Macrophage Colony Stimulating Factor (MCSF) is a hematopoietic growth factor that is widely produced by a variety of cells. MCSF stimulates the proliferation and differentiation of hematopoietic stem cells into monocyte and macrophage cell types. MCSF also acts through the colony stimulating factor 1 receptor (CSF1R) to modulate processes involved in immunology, bone metabolism, fertility, and pregnancy. Human MCSF shows activity on mouse cells, however mouse MCSF shows no activity on human cells.

Activity Assay

Human MCSF is fully biologically active when compared to standard. The activity is determined by the ability to induce NFS-60 cells proliferation.

AA Sequence

MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS

Alternative Names

Macrophage Colony Stimulating Factor, M-CSF, MGI-IM, CSF-1
Product Details
Length 159 / 218 aa
Molecular Weight 18.5 / 37.1 kDa
Structure Dimer
Source E. coli
Accession Number P09603
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 8.0
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.

Citations

Thomas L, Rao Z, Gerstmeier J, Raasch M, Weinigel C, Rummler S, Menche D, Muller R, Pergola C, Mosig A, et al. Selective upregulation of TNFα expression in classically-activated human monocyte-derived macrophages (M1) through pharmacological interference with V-ATPase. (2017). Biochem Pharmacol 130: 71-82.

Lai HY, Hsu LW, Tsai HH, Lo YC, Yang SH, Liu PY, Wang JM. CCAAT/enhancer-binding protein delta promotes intracellular lipid accumulation in M1 macrophages of vascular lesions. (2017). Cardiovascular Res 113(11): 1376-1388.

Jiang W, St-Pierre S, Roy P, Morley BJ, Hao J, Simard AR. Infiltration of CCR2+Ly6Chigh Proinflammatory Monocytes and Neutrophils into the Central Nervous System Is Modulated by Nicotinic Acetylcholine Receptors in a Model of Multiple Sclerosis. (2016). J Immunol 196(5): 2095-2108.

St-Pierre S, Jiang W, Roy P, Champigny C, LeBlanc E, Morley BJ, Hao J, Simard AR. Nicotinic Acetylcholine Receptors Modulate Bone Marrow-Derived Pro-Inflammatory Monocyte Production and Survival. (2016). PLoS One 11(2).

Sohn HM, Ko Y, Park M, Kim B, Park JE, Kim D, Moon YL, Lim W. Comparison of the alendronate and irradiation with a light-emitting diode (LED) on murine osteoclastogenesis. (2016). Lasers Med Science 32(1): 189-200.

Sohn H, Ko Y, Park M, Kim D, Moon YL, Jeong YJ, Lee H, Moon Y, Jeong BC, Kim O, et al. Effects of light-emitting diode irradiation on RANKL-induced osteoclastogenesis. (2015). Lasers Surg Med 47(9): 745-755.