PODS™ TechnologyPODS™ proteins are made using an insect cell expression system in which the active protein is co-expressed alongside polyhedrin carrier protein. Polyhedrin forms microcrystals within insect cells which specifically capture the active protein to form a co-crystal complex. The active protein is captured in its nascent, natively folded form with limited scope for proteolytic degradation. Consequently, excellent levels of bioactivity are observed. The PODS™ crystals provide a sustained release mechanism and can be used to functionalize surfaces. For further details, please refer to the PODS™ Technology page.
Product DescriptionPODS™ Empty crystals are composed solely of polyhedrin protein which self-assembles into regular, cubic crystals. Thus, these PODS™ crystals do not contain any cargo protein, in contrast to other PODS™ growth factor products, and are intended to be used as negative controls alongside cargo-containing PODS™ crystals. The cross-reactivity of PODS™ Empty crystals has been tested on a variety of cells including mouse ES cells, PC12, fibroblasts, and ETS embryos, and there was no observable interference with the tested cells. Additionally, in-vivo data indicate no inflammatory response to PODS™ Empty crystals in animals.
Usage RecommendationPODS™ Empty crystals display the same physical properties as other PODS™ growth factor products. While PODS™ Empty behave in the same way as other PODS™ crystals, they differ in that they do not contain or release cargo protein. They can be used analogous to other PODS™ growth factor products.
AA SequenceMADVAGTSNRD FRGREQRLFNS EQYNYNNSLNG EVSVWVYAYYS DGSVLVINKNS QYKVGISETFK ALKEYREGQHN DSYDEYEVNQS IYYPNGGDARK FHSNAKPRAIQ IIFSPSVNVRT IKMAKGNAVSV PDEYLQRSHPW EATGIKYRKIK RDGEIVGYSHY FELPHEYNSIS LAVSGVHKNPS SYNVGSAHNVM DVFQSCDSALR FCNRYWAELEL VNHYISPNAYP YLYINNHSYGV ALSNRQRLLV
Alternative NamesBombyx mori cypovirus polyhedrin protein
|Molecular Weight||29 kDa|
|Source||Spodoptera frugiperda (Sf9) cell culture|
|Endotoxin Level||<0.06 EU/ml as measured by gel clot LAL assay|
|Formulation||PODS™ were lyophilized from a volatile solution|
PODS™ protein crystals may be reconstituted at 200 million PODS™/ml in water. 20% glucose has a buoyant density closer to PODS™ protein crystals and can be useful for aliquoting.
PODS™ protein crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
|Stability and Storage||Upon receipt, store at 4°C. PODS™ protein crystals are stable for at least 1 year when dry and 6 months when resuspended.|