PODS™ Empty

Code Description Price Qty
PPH302-50 PODS™ Empty, 50 million £95.00
PODS™ co-crystals
PODS™ co-crystals

PODS™ Technology

PODS™ proteins are made using an insect cell expression system in which the active protein is co-expressed alongside polyhedrin carrier protein. Polyhedrin forms microcrystals within insect cells which specifically capture the active protein to form a co-crystal complex. The active protein is captured in its nascent, natively folded form with limited scope for proteolytic degradation. Consequently, excellent levels of bioactivity are observed. The PODS™ co-crystals provide a sustained release mechanism and can be used to functionalize surfaces. For further details, please refer to the PODS™ Technology page.

Product Description

PODS™ Empty crystals are composed solely of polyhedrin protein which self-assembles into regular, cubic crystals. Thus, these PODS™ co-crystals do not contain any cargo protein, in contrast to other PODS™ growth factor products, and are intended to be used as negative controls alongside cargo-containing PODS™ co-crystals. The cross-reactivity of PODS™ Empty crystals has been tested on a variety of cells including mouse ES cells, PC12, fibroblasts, and ETS embryos, and there was no observable interference with the tested cells. Additionally, in-vivo data indicate no inflammatory response to PODS™ Empty crystals in animals.

Usage Recommendation

PODS™ Empty crystals display the same physical properties as other PODS™ growth factor products. While PODS™ Empty behave in the same way as other PODS™ co-crystals, they differ in that they do not contain or release cargo protein. They can be used analogous to other PODS™ growth factor products.

AA Sequence

MADVAGTSNRD FRGREQRLFNS EQYNYNNSLNG EVSVWVYAYYS DGSVLVINKNS QYKVGISETFK ALKEYREGQHN DSYDEYEVNQS IYYPNGGDARK FHSNAKPRAIQ IIFSPSVNVRT IKMAKGNAVSV PDEYLQRSHPW EATGIKYRKIK RDGEIVGYSHY FELPHEYNSIS LAVSGVHKNPS SYNVGSAHNVM DVFQSCDSALR FCNRYWAELEL VNHYISPNAYP YLYINNHSYGV ALSNRQRLLV

Alternative Names

Bombyx mori cypovirus polyhedrin protein
Product Details
Length 252 aa
Molecular Weight 29 kDa
Structure n/a
Source Spodoptera frugiperda (Sf9) cell culture
Accession Number D37771.1
Endotoxin Level <0.06 EU/ml as measured by gel clot LAL assay
Formulation PODS™ were lyophilized from a volatile solution
Reconstitution

PODS™ co-crystals may be reconstituted at 200 million co-crystals/ml in water. 20% glucose has a buoyant density closer to PODS™ co-crystals and can be useful for aliquoting.

PODS™ co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).

Stability and Storage Upon receipt, store at 4°C. PODS™ co-crystals are stable for at least 1 year when dry and 6 months when resuspended.

References

Fasséli Coulibaly, Elaine Chiu, Keiko Ikeda, Sascha Gutmann, Peter W. Haebel, Clemens Schulze-Briese, Hajime Mori, and Peter Metcalf. The molecular organization of cypovirus polyhedra. (2007) Nature. 446: 97-101.

Rey FA. Virology: Holed up in a natural crystal. (2007) Nature. 446: 35-37.

Mori H. Immobilization of Bioactive Growth Factors into Cubic Proteinous Microcrystals (Cypovirus Polyhedra) and Control of Cell Proliferation and Differentiation. (2010) NSTI-Nanotech. 3: 222-225.

Satoshi Abe, Hiroshi Ijiri, Hashiru Negishi, Hiroyuki Yamanaka, Katsuhito Sasaki, Kunio Hirata, Hajime Mori, and Takafumi Ueno. Design of Enzyme-Encapsulated Protein Containers by In-Vivo Crystal Engineering. (2015) Advanced Materials. 27(48): 7951-7956.