PODS® Empty
PODS® Technology
PODS® proteins are made using an insect cell expression system in which the active protein is co-expressed alongside polyhedrin carrier protein. Polyhedrin forms microcrystals within insect cells which specifically capture the active protein to form a co-crystal complex. The active protein is captured in its nascent, natively folded form with limited scope for proteolytic degradation. Consequently, excellent levels of bioactivity are observed. The PODS® co-crystals provide a sustained release mechanism and can be used to functionalize surfaces. For further details, please refer to the PODS® Technology page.Product Description
PODS® Empty crystals are composed solely of polyhedrin protein which self-assembles into regular, cubic crystals. Thus, these PODS® co-crystals do not contain any cargo protein, in contrast to other PODS® growth factor products, and are intended to be used as negative controls alongside cargo-containing PODS® co-crystals. The cross-reactivity of PODS® Empty crystals has been tested on a variety of cells including mouse ES cells, PC12, fibroblasts, and ETS embryos, and there was no observable interference with the tested cells. Additionally, in-vivo data indicate no inflammatory response to PODS® Empty crystals in animals.Usage Recommendation
PODS® Empty crystals display the same physical properties as other PODS® growth factor products. While PODS® Empty behave in the same way as other PODS® co-crystals, they differ in that they do not contain or release cargo protein. They can be used analogous to other PODS® growth factor products.Animal-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.AA Sequence
MIMADVAGTS NRDFRGREQR LFNSEQYNYN SSLNGEVSVW VYAYYSDGSV LVINKNSQYK VGISETFKAL KEYREGQHND SYDEYEVNQS IYYPNGGDAR KFHSNAKPRA IQIIFSPSVN VRTIKMAKGN AVSVPDEYLQ RSHPWEATGI KYRKIKRDGE IVGYSHYFEL PHEYNSISLA VSGVHKNPSS YNVGSAHNVM DVFQSCDLAL RFCNRYWAEL ELVNHYISPN AYPYLDINNH SYGVALSNRQAlternative Names
Bombyx mori cypovirus polyhedrin proteinResearch Use Only
This product is for Research Use Only.Product Details | |
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Length | 250 aa |
Molecular Weight | 28.7 kDa |
Structure | n/a |
Source | Spodoptera frugiperda (Sf9) cell culture |
Accession Number | D37771.1 |
Endotoxin Level | <0.06 EU/ml as measured by gel clot LAL assay |
Formulation | PODS® were lyophilized from a volatile solution |
Reconstitution |
PODS® co-crystals may be reconstituted at 200 million co-crystals/ml in sterile PBS. 20% glucose has a buoyant density closer to PODS® co-crystals and can be useful for aliquoting. PODS® co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8). |
Stability and Storage | Upon receipt, store at 4°C. PODS® co-crystals are stable for at least 1 year when dry and 6 months when resuspended. |
References
Fasséli Coulibaly, Elaine Chiu, Keiko Ikeda, Sascha Gutmann, Peter W. Haebel, Clemens Schulze-Briese, Hajime Mori, and Peter Metcalf. The molecular organization of cypovirus polyhedra. (2007) Nature. 446: 97-101.
Rey FA. Virology: Holed up in a natural crystal. (2007) Nature. 446: 35-37.
Mori H. Immobilization of Bioactive Growth Factors into Cubic Proteinous Microcrystals (Cypovirus Polyhedra) and Control of Cell Proliferation and Differentiation. (2010) NSTI-Nanotech. 3: 222-225.
Satoshi Abe, Hiroshi Ijiri, Hashiru Negishi, Hiroyuki Yamanaka, Katsuhito Sasaki, Kunio Hirata, Hajime Mori, and Takafumi Ueno. Design of Enzyme-Encapsulated Protein Containers by In-Vivo Crystal Engineering. (2015) Advanced Materials. 27(48): 7951-7956.