Exo-spin isolation service

Exo-spin

Simple, easy-to-use products and protocols for reproducible results. Protocol overview. (1) The sample is spun to remove cells and (optional step) precipitated to concentrate exosomes. (2) The column closures are removed (3) the column is equilibrated (4) the sample is added (5) purified exosomes are eluted  

Exosome purification using size exclusion chromatography (SEC)

SEC separates particles in solution based on their size. An SEC column is packed with resin containing stable polymeric spherical beads, each of which has large numbers of nano-scale pores across its surface. The beads leave gaps after packing, the size of which depends on the diameter of the beads. When a solution containing exosomes is added to the top of the resin bed, the smallest particles, including free-proteins, become trapped in the nano-sized pores, slowing their progress, whereas the larger exosomes pass around the outside of the beads and elute first. The very largest particles, beyond the size of the gaps between the beads, are also slowed or stopped completely. The resin used in Exo-spin columns has been chosen to specifically allow primary elution of particles in the range 30nm - 250nm. This allows the isolation of exosomes from particles of other sizes.