PeptiInk® Alpha 4 PLUS™
Synthetic Self-Assembling Peptide Bioinks
PeptiInks® are specifically designed peptide hydrogels for liquid handling and 3D bioprinting cell culture applications. The PeptiInk® family of bioinks are shear thinning and can be used for 3D bioprinting with any extrusion-based printer. Cells can be encapsulated and printed directly with structural definition and long-term viability and stability.
PeptiInks® are linked to the PeptiGel® series, allowing you to continue using the most suitable product (e.g. Alpha 4 PLUS) for your cells when you transition from bench to printed 3D cell culture.
Product Description
PeptiInk® Alpha 4 PLUS™ is a ready-to-use fully synthetic positively charged peptide bioink functionalized with fibronectin (RGD) and collagen (GFOGER) motifs, mimicking the extracellular matrix more closely and encouraging better cellular adhesion. PeptiInk® Alpha 4 PLUS™ is suitable for the culture of a wide variety of cells, including primary cells, immortalized cell lines and stem cells.
PeptiInk® Alpha 4 PLUS™ is highly consistent in mechanical stiffness (G’) and pore size, resulting in excellent reproducible results. Hence, PeptiInk® Alpha 4 PLUS™ can be used for both in vitro and in vivo applications with physiologically and clinically relevant results.
Value Starter Pack
When beginning to work with PeptiGels®, we recommend purchasing our Value Starter Pack. This includes PeptiGel® to test with your cells to enable you to identify the most suitable matrix environment. The Pack also includes a Gilson positive displacement pipette (various sizes are available), a box of sterile tips and a pack of Greiner Thincerts. Contact us for additional information.
Case Studies
PeptiInk® supports complex structures laden with cells
PeptiInk® successfully prints complex structures laden with mammalian epithelial cells using a commercially available extrusion bioprinter. Structures could be printed with high structural integrity and geometrical/dimensional accuracy. After 7 day culture, the number of viable cells remained high and began to proliferate.
References
- Clough HC, O'Brien M, Zhu X, Miller AF, Saiani A, Tsigkou O. (2021) Neutrally charged self-assembling peptide hydrogel recapitulates in vitro mechanisms of breast cancer progression. Materials Science and Engineering 127: 112200
| Product Details | |
|---|---|
| Formulation |
Synthetic, self-assembling peptide bioink |
| Charge |
Positive (+2) |
| pH |
6.2 - 7.2 |
| Mechanical Properties (kPa) |
0.7 - 1.3 |
| Biocompatibility |
Non-toxic, safe for animal studies |
| Biodegradability |
Biodegradable, supports cell activities |
| Functional motif: GFOGER (Collagen) |
Yes |
| Functional motif: RGD (Fibronectin) |
Yes |
| Stability and Storage |
At least 6 months from receipt when stored at 4°C as supplied. |
| Shipping Temperature |
Ambient |
