PODS universal cargo antibody (PUCA)
PODS universal cargo antibody
Description
The PODS universal cargo antibody (PUCA) binds to the hinge region between the H! affinity tag and the cargo molecules. It has been developed by Cell Guidance Systems to act as a universal antibody to detect and quantity cargo molecules released from PODS crystals.
Product Usage Information
Western blotting: Dilution 1:5000
Storage
Supplied in PBS + 0.02% ProClin 300. Store at -20oC
Source
The monoclonal antibody was produced by immunizing animals with a synthetic peptide
corresponding to the H1 capture tag.
Specificity
The universal cargo antibody detects the H1 capture tag KNSRPSTSLYKKAGF
Product data
Lane 1 = Empty PODS
Lane 2 = PODS human SCF
Western blot analysis of empty and PODS human SCF
