ExoLISA Assays

Tools for discovery

Exosomes are complex variable structures which require basic characterization as part of any study. Guidance on the minimal level of characterization that should be performed before a study can be published has been provided by the exosome community.  Cell Guidance Systems has developed a range of solutions based on exosome composition and characteristics. These include: exosome marker antibodies, ExoLISA™ detection assay, Instant Exosomes™ and we also offer an NTA size profiling service.

ExoLISA™ technology: exosome detection

The ExoLISA™ exosome detection assay is an ELISA-like assay in the sense that it is an antibody sandwich-based assay for the detection of exosome particles. However, there are some significant differences. Unlike a standard ELISA assay, there is no enzymatic reaction. Rather, the exosome target is detected directly with a Europium labelled-antibody, constituting a time-resolved immunofluorescence assay.

The ExoLISA™ exosome assays provide clear and consistent data quickly from purified or unpurified samples in a convenient 96-well format. The kit is available for marker proteins CD9, CD63, and CD81.