Tools for discovery
Exosomes are complex variable structures which require basic characterization as part of any study. Guidance on the minimal level of characterization that should be performed before a study can be published has been provided by the exosome community. Cell Guidance Systems has developed a range of solutions based on exosome composition and characteristics. These include: exosome marker antibodies, ExoLISA™ detection assay, Instant Exosomes™ and we also offer an NTA size profiling service.
ExoLISA™ technology: exosome detection
The ExoLISA™ exosome detection assay is an ELISA-like assay in the sense that it is an antibody sandwich-based assay for the detection of exosome particles. However, there are some significant differences. Unlike a standard ELISA assay, there is no enzymatic reaction. Rather, the exosome target is detected directly with a Europium labelled-antibody, constituting a time-resolved immunofluorescence assay.
The ExoLISA™ exosome assays provide clear and consistent data quickly from purified or unpurified samples in a convenient 96-well format. The kit is available for marker proteins CD9, CD63, and CD81.