Exo-spin™

Code Description Price Qty
EX01-8 Exo-spin™ kit, 8 columns, 60 ml buffer £105.00
EX01-25 Exo-spin™ kit, 24 columns, 250 ml buffer £300.00
EX01-25L Exo-spin™ kit, 24 columns, 500 ml buffer £355.00
EX01-50 Exo-spin™ kit, 48 columns, 500 ml buffer £540.00
EX06-30 Exo-spin™ buffer, 30 ml £35.00
EX06-250 Exo-spin™ buffer, 250 ml £160.00
EX03-8 Exo-spin™ columns, 8 columns only £60.00
EX03-25 Exo-spin™ columns, 24 columns only £155.00
EX03-50 Exo-spin™ columns, 48 columns only £245.00
Exo-spin™ columns
Exo-spin™ buffer

Product description

Exo-spin™ products provide a proven, rapid and reliable way of generating high quality purified exosomes suitable for a range of research applications.

Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), making it superior to other techniques that rely solely on one method. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. We provide a simple, two-step protocol which allows consistent and reliable purification of samples in under 2 hours.

Each column in the EX01 Exo-spin™ kit enables processing of up to 50 ml of cell culture medium, saliva, urine, cerebrospinal fluid (CSF), human breast milk and other low-exosome-content biological fluids. 

Products on this page contain Exo-spin™ mini columns

 

Which types of sample can I use with EX01 Exo-spin™ kit? Please see here for other sample types

 

 

Low exosome-content biofluids (up to 1x109/ml)

including cell culture media, saliva, cerebrospinal fluid, urine

Sample Volume

isolation method

Exo-spin Kit

column bed length

< 1ml to 50 ml 

concentration + SEC

EX01 mini

1.3 cm 

 < 1 ml to 75 ml Note 1

concentration + SEC

EX05 mini-HD

6.35 cm

75 ml to 500 ml Note 1 concentration + SEC EX04 midi 2.15 cm

 


* For cerebrospinal fluid (CSF) and human breast milk samples, validated protocols are available for EX01 only. The protocols are provided in the user guides.
 

Exo-spin™ column and resin pore size

The  Exo-spin™ mini-column bed volume is 500 µl, allowing 100 µl of volume to be loaded on top of the column.

The resin used in these columns contains pores with a diameter of approximatively 30 nm.  All molecules which are smaller than 30 nm (e.g. free proteins, lipids) will enter into the pores and have their progress through the resin slowed. Using the protocol provided, these smaller particles remain trapped in the resin. Particles larger than 30nm that are still small enough to fit between the resin beads elute first. The first fraction to elute from the column contains particles between 30-300nm.

Highest recovery and purity

Essentially all proteins and lipids are retained in the column ensuring a highly pure sample ready for your downstream application.

Reproducibility

All our columns are manufactured in our manufacturing facility to ensure a high reproducibility between each lot. A large number of peer-reviewed scientific papers have been published describing the use of Exo-spin™.

Storage

Upon receipt, store purification Exo-spin™columns and Exo-spin™ Buffer should be stored at 4°C. All other components should be stored at room temperature (15°C - 25°C).

Frequently Asked Questions (FAQs)

For any additional questions, please refer to FAQs document below.

Start today! Select our starter pack

We designed the ideal starter pack to guide your exosome research. The starter pack includes the exosome purification kit of your choice, exosome validated antibodies, and NTA profiling analysis. The complete details can be found in the product page here.

 

Product data

EX01 Exo-spin™ kit comparative data

NTARNA analysis

NTA measurement of samples prepared from cell culture medium: (a) Exo-spin™ yields significantly higher numbers of vesicles of the expected 40-120 nm size range than alternatives including (b) ultracentrifugation (c) competitor kit. The size distribution profile obtained with Exo-spin™ most closely resembles ultra-centrifugation. Lower rRNA contamination levels Analysis on Bioanalyzer instrument shows RNA preps (Trizol) following (d) Exo-spin™ purification from cell culture samples compared with (e) ultracentrifugation or (f) competitor kit.
Exosomes from CSF
Data determined by nanoparticle tracking analysis (NTA). Each curve represents the average of 3 technical replicate measurements for each exosome isolation method triplicate experiment. (PM = Precipitation Method). Figure taken and adapted from (Martins, TS et al., 2018).

 

Characterizing Exo-spin™ isolated exosomes using nanoparticle tracking analysis (NTA)

Nanoparticle Tracking Analysis NTA

Exosome characterization with NTA. Exosomes have been isolated using the Exo-spin™ kit and analysis performed with the ZetaView® instrument.

Downstream applications: advice and content

The EX01 Exo-spin™ kit is compatible with all downstream application and has been published in a large range of different applications.

RNA analysis:

  • Li Z, Mbah NE and Maltese WA. (2018). Vacuole-inducing compounds that disrupt endolysosomal trafficking stimulate production of exosomes by glioblastoma cells. Molecular and Cellular Biochemistry 439(1-2):1-9.
  • Koo KM, Wee EJ and Trau M.; (2016). Colorimetric TMPRSS2-ERG Gene Fusion Detection in Prostate Cancer Urinary Samples via Recombinase Polymerase Amplification. Theranostics 6(9): 1415–1424.
  • Yoon C, Kim J, Park G, Kim S, Kim D, Hur DY, Kim B and Kim YS. (2016). Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt's lymphoma exosomes. Tumor Biology 37(1):313-21.

Exosome engineering for therapeutic cargo:

  • Kojima R, Bojar D, Rizzi G, Hamri GC, El-Baba MD, Saxena P, Ausländer S, Tan KR and Fussenegger M. (2018). Designer exosomes produced by implanted cells intracerebrally deliver therapeutic cargo for Parkinson's disease treatment. Nature communications 9: 1305.

Functional study:

  • Piao YJ, Kim HS, Hwang EH, Woo J, Zhang M and Moon WK. (2017). Breast cancer cell-derived exosomes and macrophage polarization are associated with lymph node metastasis. Oncotarget 9(7): 7398–7410.

Proteomic (MS/MS analysis):

  • E L Kavanagh, S Lindsay, M Halasz, L C Gubbins, K Weiner-Gorzel, M H Z Guang, A McGoldrick, E Collins, M Henry, A Blanco-Fernández, P O' Gorman, P Fitzpatrick, M J Higgins, P Dowling, and A McCann. (2017). Protein and chemotherapy profiling of extracellular vesicles harvested from therapeutic induced senescent triple negative breast cancer cells. Oncogenesis 6(10): e388.

Exosome labelling:

  • Lara P, Palma-Florez S, Salas-Huenuleo E, Polakovicova I, Guerrero S, LobosGonzalez L, Campos A, Muñoz L, Jorquera-Cordero C, Varas-Godoy M, Cancino J, Arias E, Villegas J, Cruz LJ, Albericio F, Araya E, Corvalan AH, Quest AFG, Kogan MJ (2020). Gold nanoparticle based double-labeling of melanoma extracellular vesicles to determine the specificity of uptake by cells and preferential accumulation in small metastatic lung tumors. Journal of Nanobiotechnology 23;18(1):20.

Application note:

 

Citations

Li Z, Mbah NE and Maltese WA. (2018). Vacuole-inducing compounds that disrupt endolysosomal trafficking stimulate production of exosomes by glioblastoma cells. Molecular and Cellular Biochemistry 439(1-2):1-9.

Kojima R, Bojar D, Rizzi G, Hamri GC, El-Baba MD, Saxena P, Ausländer S, Tan KR and Fussenegger M. (2018). Designer exosomes produced by implanted cells intracerebrally deliver therapeutic cargo for Parkinson's disease treatment. Nature communications 9: 1305.

Piao YJ, Kim HS, Hwang EH, Woo J, Zhang M and Moon WK. (2017). Breast cancer cell-derived exosomes and macrophage polarization are associated with lymph node metastasis. Oncotarget 9(7): 7398–7410.

E L Kavanagh, S Lindsay, M Halasz, L C Gubbins, K Weiner-Gorzel, M H Z Guang, A McGoldrick, E Collins, M Henry, A Blanco-Fernández, P O' Gorman, P Fitzpatrick, M J Higgins, P Dowling, and A McCann. (2017). Protein and chemotherapy profiling of extracellular vesicles harvested from therapeutic induced senescent triple negative breast cancer cells. Oncogenesis 6(10): e388.

Koo KM, Wee EJ and Trau M.; (2016). Colorimetric TMPRSS2-ERG Gene Fusion Detection in Prostate Cancer Urinary Samples via Recombinase Polymerase Amplification. Theranostics 6(9): 1415–1424.

Yoon C, Kim J, Park G, Kim S, Kim D, Hur DY, Kim B and Kim YS. (2016). Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt's lymphoma exosomes. Tumor Biology 37(1):313-21.