Karyotype Service for Mouse Cells

Code Description Price Qty
K05 Karyotype service from fixed mouse cells £350.00
K06 Karyotype service from live mouse cells, BSL1 £425.00
K08 Karyotype service from live mouse cells, BSL2 £475.00

Service Description

All services aim to provide karyotype analysis of 20 G-banded metaphase spreads from a single cell culture sample. If fewer than 20 cells are found in the sample provided, as many cells as possible will be analyzed. At least one high-quality image of a representative karyotype is provided which is suitable for publication. In the event karyotypic abnormalities are found, additional figures will be provided. If complex mosaic karyotypes are found, fewer than 20 cells may be analyzed.

Please note that for cancer cells and in any cases where very complex, unstable karyotypes are observed, a full karyotype for a single cell will be provided. Additional charges will apply to any additional cell analysis.

For each species, there are two levels of service available:

(1) Cells growing in culture (which we process for analysis),

(2) Cells already processed and fixed in your lab (we provide a detailed protocol).

The results are returned within 20/22 business days for mouse samples.

 

Citations

Tidball AM, Dang LT, Glenn TW, Kilbane EG, Klarr DJ, Margolis JL, Uhler MD, and Parent JM. Rapid Generation of Human Genetic Loss-of-Function iPSC Lines by Simultaneous Reprogramming and Gene Editing. (2017). Stem Cell Reports 9(3): 725-731.

Ludtmann MHR, Arber C, Bartolome F, de Vicente M, Preza E, Carro E, Houlden H, Gandhi S, Wray S, and Abramov AY. Mutations in valosin-containing protein (VCP) decrease ADP/ATP translocation across the mitochondrial membrane and impair energy metabolism in human neurons. (2017). Journal of Biological Chemistry 292(21): 8907-8917.

Zhang Y, Schmid B, Nielsen TT, Nielsen JE, Clausen C, Hyttel P, Holst B, and Freude KK. Generation of a human induced pluripotent stem cell line via CRISPR-Cas9 mediated integration of a site-specific heterozygous mutation in CHMP2B. (2016). Stem Cell Research 17(1): 148-150.

Chen H, Aksoy I, Gonnot F, Osteil P, Aubry M, Hamela C, Rognard C, Hochard A, Voisin S, Fontaine E, et al. Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency. (2015). Nature Communications 6: 7095.