Karyotype Service for Mouse Cells

Code Description Price Qty
K05 Karyotype service from fixed mouse cells £350.00
K06 Karyotype service from live mouse cells, BSL1 £425.00
K08 Karyotype service from live mouse cells, BSL2 £475.00

Service description

The service results consist of a descriptive report and at least one high-quality image of a representative karyotype.

For all services, we aim to provide karyotype analysis of 20 G-banded metaphase spreads from a single-cell culture sample. However, if fewer than 20 cells are found in the sample provided, as many cells as possible will be analyzed.

For complex mosaic karyotypes, fewer than 20 cells may be analyzed and additional karyogram images will be provided in the report. Please note that for cancer cells and in any cases where very complex, unstable karyotypes are observed, the analysis consists only of a full karyotype for a single cell. If further analysis is required, additional charges will apply.

The results are returned by email within 20–22 business days for mouse samples. Please note that lead times can be longer during busier periods. If this applies to your order, our team will keep you updated.

 

Types of service

There are three options available:

  • Karyotype service from fixed mouse cells – cells processed and fixed in the researchers’ laboratories. A complete, detailed protocol is provided.
  • Karyotype service from live mouse cells, BSL1 – we receive live cells in culture, which we process in our laboratories.
  • Karyotype service from live mouse cells, BSL2 – we receive live cells in culture, which we process in our laboratories. A risk assessment is created by our team, so an extra questionnaire is provided.

In order to accept and process live cells that are classed as BSL2, we require evidence of the following:

  • Confirmation via PCR or equivalent test that the sample material is free of human pathogens (HBV, HCV, HIV-1, HIV-2, HTLV I/II, CMV, EBV, HSV-1, HSV-2) from a CLIA-certified lab. 
  • Confirmation via PCR or equivalent that the sample is free of mycoplasma.

This screening is not necessary for the receipt of fixed samples.

 

If you have any questions, please contact us by email at info@cellgs.com. We will respond within one business day.

Please do not send any samples without notice.

Karyogram images

Example karyogram of a mouse stem cell line with a normal karyotype 40,XY.

 

Example karyogram of a mouse stem cell line with an abnormal karyotype 41,XY.

 

Citations

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Schöndorf DC, Elschami M, Schieck M, Ercan-Herbst E, Weber C, Riesinger Y, Kalman S, Steinemann D, and Ehrnhoefer DE. Generation of an induced pluripotent stem cell cohort suitable to investigate sporadic Alzheimer's Disease. (2018). Stem Cell Research Jan;34:101351.

Joshi PS, Modur V, Cheng J, Robinson K and Rao K. Characterization of immortalized human mammary epithelial cell line HMEC 2.6. (2017). Tumour biology Oct;39(10):1010428317724283.

Tidball AM, Dang LT, Glenn TW, Kilbane EG, Klarr DJ, Margolis JL, Uhler MD, and Parent JM. Rapid Generation of Human Genetic Loss-of-Function iPSC Lines by Simultaneous Reprogramming and Gene Editing. (2017). Stem Cell Reports 9(3): 725-731.

Ludtmann MHR, Arber C, Bartolome F, de Vicente M, Preza E, Carro E, Houlden H, Gandhi S, Wray S, and Abramov AY. Mutations in valosin-containing protein (VCP) decrease ADP/ATP translocation across the mitochondrial membrane and impair energy metabolism in human neurons. (2017). Journal of Biological Chemistry 292(21): 8907-8917.

Zhang Y, Schmid B, Nielsen TT, Nielsen JE, Clausen C, Hyttel P, Holst B, and Freude KK. Generation of a human induced pluripotent stem cell line via CRISPR-Cas9 mediated integration of a site-specific heterozygous mutation in CHMP2B. (2016). Stem Cell Research 17(1): 148-150.

Chen H, Aksoy I, Gonnot F, Osteil P, Aubry M, Hamela C, Rognard C, Hochard A, Voisin S, Fontaine E, et al. Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency. (2015). Nature Communications 6: 7095.