PeptiGel® Alpha 4™

Code Description Price Qty
PA4-5 PeptiGel® Alpha 4™, 5 ml $395.00
PA4-20 PeptiGel® Alpha 4™, 20 ml $1,390.00
PA4-50 PeptiGel® Alpha 4™, 50 ml $2,990.00
PA4-SP PeptiGel® Alpha 4™ Starter Pack $1,095.00
PeptiGel® Alpha 4™

Synthetic Self-Assembling Peptide Hydrogels

PeptiGels® are ready-to-use fully synthetic self-assembling peptide hydrogels that are biologically relevant and formulated to meet your cells’ needs. PeptiGels® are used for 2D and 3D cell culture and are specifically designed with a range of mechanical properties and chemical functionalities to mimic the native cellular micro-environment to allow the growth of any cell type.

Product Description

PeptiGel® Alpha 4™ is a ready-to-use fully synthetic positively charged peptide hydrogel. PeptiGel® Alpha 4™ is biologically relevant, mimics the extracellular matrix and is suitable for the culture of a variety of cells, including primary cells, immortalized cell lines and stem cells.

PeptiGel® Alpha 4™ is highly consistent in mechanical stiffness (G’) and pore size, resulting in excellent reproducible results. Hence, PeptiGel® Alpha 4™ can be used for both in vitro and in vivo applications with physiologically and clinically relevant results.

Value Starter Pack

When beginning to work with PeptiGels®, we recommend purchasing our Value Starter Pack. This includes PeptiGel® to test with your cells to enable you to identify the most suitable matrix environment. The Pack also includes a Gilson positive displacement pipette (various sizes are available), a box of sterile tips and a pack of Greiner Thincerts. Contact us for additional information.

Case Studies

PeptiGel® supports the growth of kidney organoids

PeptiGel Alpha 4 Kidney Organoid

 

PeptiGel® Alpha 4™ successfully supports the growth of kidney organoids. Kidney organoids grown on PeptiGel® were shown to express key markers of renal differentiation including LTL+ve proximal tubules, ECAD+ve distal tubule/collecting duct and ZO-1+ve tight junctions. These cell types were supported by Meis1/2/3+ve interstitial cells and Laminin+ve basement membrane.

 

PeptiGel® supports the growth of in-vitro ovarian cancer model

PeptiGel Alpha 4 Ovarian Cancer Model

PeptiGel® Alpha 4™ successfully supports the growth of an in-vitro epithelial ovarian cancer model. Analysis of cell viability across different PeptiGels® showed that Alpha 4-GFOGER™ (collagen functionalized) facilitated earlier cellular growth than Alpha 4™ and Alpha 4-RGD™ (fibronectin functionalized). However, after 28 days all hydrogels supported the growth of ovarian cancer cells.

References

  • Clough HC, O'Brien M, Zhu X, Miller AF, Saiani A and Tsigkou O. (2021) Neutrally charged self-assembling peptide hydrogel recapitulates in vitro mechanisms of breast cancer progression. Materials Science and Engineering 127: 112200.
  • Treacy NJ, Clerkin S, Davis JL, Kennedy C, Miller AF, Saiani A, Wychowaniec JK, Brougham DF and Crean J. (2023) Growth and differentiation of human induced pluripotent stem cell (hiPSC)-derived kidney organoids using fully synthetic peptide hydrogels. Bioactive Materials 21, 142-156.
  • King K, Cartwright E, Oceandy D, Zi M, Facchi C and Njegic A. (2022) BS43 Pro-angiogenic injectable self-assembling peptide hydrogel as a delivery vehicle for cardiac cell therapies. Heart 108, A171-A172.
Product Details
Formulation

Synthetic, self-assembling peptide hydrogel

Charge

Positive (+2)

pH

6.8 - 7.2

Mechanical Properties (kPa)

0.7 - 1.3

Biocompatibility

Non-toxic, safe for animal studies  

Biodegradability

Biodegradable, supports cell activities

Functional motif: GFOGER (Collagen)

No

Functional motif: RGD (Fibronectin)

No

Stability and Storage

At least 6 months from receipt when stored at 4°C as supplied.

Shipping Temperature

Ambient