PeptiGel® Alpha 4™
Synthetic Self-Assembling Peptide Hydrogels
PeptiGels® are ready-to-use fully synthetic self-assembling peptide hydrogels that are biologically relevant and formulated to meet your cells’ needs. PeptiGels® are used for 2D and 3D cell culture and are specifically designed with a range of mechanical properties and chemical functionalities to mimic the native cellular micro-environment to allow the growth of any cell type.
Product Description
PeptiGel® Alpha 4™ is a ready-to-use fully synthetic positively charged peptide hydrogel. PeptiGel® Alpha 4™ is biologically relevant, mimics the extracellular matrix and is suitable for the culture of a variety of cells, including primary cells, immortalized cell lines and stem cells.
PeptiGel® Alpha 4™ is highly consistent in mechanical stiffness (G’) and pore size, resulting in excellent reproducible results. Hence, PeptiGel® Alpha 4™ can be used for both in vitro and in vivo applications with physiologically and clinically relevant results.
Case Studies
PeptiGel® supports the growth of kidney organoids
PeptiGel® Alpha 4™ successfully supports the growth of kidney organoids. Kidney organoids grown on PeptiGel® were shown to express key markers of renal differentiation including LTL+ve proximal tubules, ECAD+ve distal tubule/collecting duct and ZO-1+ve tight junctions. These cell types were supported by Meis1/2/3+ve interstitial cells and Laminin+ve basement membrane.
PeptiGel® supports the growth of in-vitro ovarian cancer model
PeptiGel® Alpha 4™ successfully supports the growth of an in-vitro epithelial ovarian cancer model. Analysis of cell viability across different PeptiGels® showed that Alpha 4-GFOGER™ (collagen functionalized) facilitated earlier cellular growth than Alpha 4™ and Alpha 4-RGD™ (fibronectin functionalized). However, after 28 days all hydrogels supported the growth of ovarian cancer cells.
References
- Clough HC, O'Brien M, Zhu X, Miller AF, Saiani A, Tsigkou O. (2021) Neutrally charged self-assembling peptide hydrogel recapitulates in vitro mechanisms of breast cancer progression. Materials Science and Engineering 127: 112200
| Product Details | |
|---|---|
| Charge |
Positive (+2) |
| Mechanical Properties (kPa) |
0.35 - 0.7 |
| Functional motif: GFOGER (Collagen) |
No |
| Functional motif: RGD (Fibronectin) |
No |
| Stability and Storage |
At least 6 months from receipt when stored at 4°C as supplied. |
