PeptiGel® Alpha 4™
Demonstrated Compatible Cells
Mammary Epithelial | Bone Marrow-derived Stem Cells | Dermal Fibroblasts | Colon Organoids | Kidney Organoids | Synovial Cells | iPSCs | Breast MCF7 | Breast MCF10a | Breast MDAMB231 | Fibroblast 3T3 | Fibroblast L929 | Muscle C2C12 | Ovary A2780 | HUVECS
Synthetic Self-Assembling Peptide Hydrogels
PeptiGels® are ready-to-use fully synthetic self-assembling peptide hydrogels that are biologically relevant and formulated to meet your cells’ needs. PeptiGels® are used for 2D and 3D cell culture and are specifically designed with a range of mechanical properties and chemical functionalities to mimic the native cellular micro-environment to allow the growth of any cell type.
Product Description
PeptiGel® Alpha 4™ is a ready-to-use fully synthetic positively charged peptide hydrogel. PeptiGel® Alpha 4™ is biologically relevant, mimics the extracellular matrix and is suitable for the culture of a variety of cells, including primary cells, immortalized cell lines and stem cells.
PeptiGel® Alpha 4™ is highly consistent in mechanical stiffness (G’) and pore size, resulting in excellent reproducible results. Hence, PeptiGel® Alpha 4™ can be used for both in vitro and in vivo applications with physiologically and clinically relevant results.
Value Starter Pack
When beginning to work with PeptiGels®, we recommend purchasing our Value Starter Pack. This includes PeptiGel® to test with your cells to enable you to identify the most suitable matrix environment. The Pack also includes a Gilson positive displacement pipette (various sizes are available), a box of sterile tips and a pack of Greiner Thincerts. Contact us for additional information.
Case Studies
PeptiGel® supports the growth of kidney organoids
PeptiGel® Alpha 4™ successfully supports the growth of kidney organoids. Kidney organoids grown on PeptiGel® were shown to express key markers of renal differentiation including LTL+ve proximal tubules, ECAD+ve distal tubule/collecting duct and ZO-1+ve tight junctions. These cell types were supported by Meis1/2/3+ve interstitial cells and Laminin+ve basement membrane.
PeptiGel® supports the growth of in-vitro ovarian cancer model
PeptiGel® Alpha 4™ successfully supports the growth of an in-vitro epithelial ovarian cancer model. Analysis of cell viability across different PeptiGels® showed that Alpha 4-GFOGER™ (collagen functionalized) facilitated earlier cellular growth than Alpha 4™ and Alpha 4-RGD™ (fibronectin functionalized). However, after 28 days all hydrogels supported the growth of ovarian cancer cells.
References
- Clough HC, O'Brien M, Zhu X, Miller AF, Saiani A and Tsigkou O. (2021) Neutrally charged self-assembling peptide hydrogel recapitulates in vitro mechanisms of breast cancer progression. Materials Science and Engineering 127: 112200.
- Treacy NJ, Clerkin S, Davis JL, Kennedy C, Miller AF, Saiani A, Wychowaniec JK, Brougham DF and Crean J. (2023) Growth and differentiation of human induced pluripotent stem cell (hiPSC)-derived kidney organoids using fully synthetic peptide hydrogels. Bioactive Materials 21, 142-156.
- King K, Cartwright E, Oceandy D, Zi M, Facchi C and Njegic A. (2022) BS43 Pro-angiogenic injectable self-assembling peptide hydrogel as a delivery vehicle for cardiac cell therapies. Heart 108, A171-A172.
| Product Details | |
|---|---|
| Formulation |
Synthetic, self-assembling peptide hydrogel |
| Charge |
Positive (+2) |
| pH |
6.8 - 7.2 |
| Mechanical Properties (kPa) |
0.7 - 1.3 |
| Biocompatibility |
Non-toxic, safe for animal studies |
| Biodegradability |
Biodegradable, supports cell activities |
| Functional motif: GFOGER (Collagen) |
No |
| Functional motif: RGD (Fibronectin) |
No |
| Stability and Storage |
At least 6 months from receipt when stored at 4°C as supplied. |
| Shipping Temperature |
Ambient |
