PeptiGel® Alpha 2™
Demonstrated Compatible Cells
Adipose-derived Stem Cells | iPSC-derived Cardiomyocytes | Chondrocytes | Dorsal Root Ganglion Neurons | Liver HepG2 | Pancreas Suit-2 | Prostate PC3 | Prostate pNT2
Synthetic Self-Assembling Peptide Hydrogels
PeptiGels® are ready-to-use fully synthetic peptide hydrogels that are biologically relevant and formulated to meet your cells’ needs. PeptiGels® are used for 2D and 3D cell culture and are specifically designed with a range of mechanical properties and chemical functionalities to mimic the native cellular micro-environment to allow the growth of any cell type.
Product Description
PeptiGel® Alpha 2™ is a ready-to-use fully synthetic positively charged peptide hydrogel. PeptiGel® Alpha 2™ is biologically relevant, mimics the extracellular matrix and is suitable for the culture of a variety of cells, including neuronal cells.
PeptiGel® Alpha 2™ is a stiffer hydrogel when compared to Alpha 4 but has consistent mechanical stiffness (G’) and pore size, resulting in excellent reproducible results. PeptiGel® Alpha 2™ can be used for both in-vitro and in-vivo applications with physiologically and clinically relevant results.
Trial Size and Value Starter Pack
When beginning to work with PeptiGels®, we recommend purchasing our 1 ml trial pack sizes for new users. This allows you to evaluate the benefits of PeptiGel and PeptiInk hydrogels in your culture system at a very low cost. New users can purchase up to five 1 ml pack sizes.
We also offer a Value Starter Pack. This includes five versions of PeptiGel® of your choice (1 ml each) to test with your cells to enable you to identify the most suitable matrix environment. The Pack also includes a Gilson positive displacement pipette (various sizes are available), a box of sterile tips and a pack of Greiner Thincerts. Contact us for additional information.
Case Studies
PeptiGel® outperforms Matrigel™
PeptiGel® Alpha 2™ supports quicker growth of fully formed gastro-intestinal organoids than Matrigel™.
Tailored PeptiGel® properties mimic the mechanical and chemical environment of healthy and cancer tissue
Tumour mimicking (stiff and acidic) PeptiGel® Alpha 2™ results in increased proliferation of Pancreatic Ductal Adenocarcinoma suit-2 cells.
PeptiGel® enables the migration of resident cells into injury
PeptiGel® Alpha 2™ implanted in the brain was well tolerated and supported the migration of resident cells into a lesion created by treatment with collagenase.
References
- Lachowski D, Matellan C, Cortes E, Saiani A, Miller AF and del Río Hernández AE. (2021) Self-assembling polypeptide hydrogels as a platform to recapitulate the tumor microenvironment. Cancers 13(13): 3286.
- King K, Cartwright E, Oceandy D, Zi M, Facchi C and Njegic A. (2022) BS43 Pro-angiogenic injectable self-assembling peptide hydrogel as a delivery vehicle for cardiac cell therapies. Heart 108, A171-A172.
- Clough HC, O'Brien M, Zhu X, Miller AF, Saiani A and Tsigkou O. (2021) Neutrally charged self-assembling peptide hydrogel recapitulates in vitro mechanisms of breast cancer progression. Materials Science and Engineering 127: 112200
| Product Details | |
|---|---|
| Formulation |
Synthetic, self-assembling peptide hydrogel |
| Charge |
Positive (+1) |
| pH |
3.8 - 4.2 |
| Mechanical Properties (kPa) |
6.0 - 8.0 |
| Biocompatibility |
Non-toxic, safe for animal studies |
| Biodegradability |
Biodegradable, supports cell activities |
| Functional motif: GFOGER (Collagen) |
No |
| Functional motif: RGD (Fibronectin) |
No |
| Stability and Storage |
At least 6 months when stored at 4°C as supplied. |
| Shipping Temperature |
Ambient |
