Orangu™ Cell Counting Solution
Orangu™ is a non-cytotoxic, sensitive, colorimetric assay for the determination of cell viability in cell proliferation and cytotoxicity assays. Orangu™ utilizes WST-8, a highly water-soluble tetrazolium salt that is reduced by dehydrogenase activities in viable cells, to give an orange-coloured formazan dye. The amount of dye formed is directly proportional to the number of living cells. Increasing incubation periods or number of cells will lead to an increase in the amount of orange dye. Simple!
Three simple steps to use Orangu™:
Step 1 Step 2 Step 3
Step 1: Add Orangu™ to your cells,
Step 2: Incubate (typically 1 - 4 hours),
Step 3: Read absorbance at 450 nm in a standard plate reader.
Orangu™ utilizes WST-8, a highly water-soluble tetrazolium salt that is reduced by dehydrogenase activities in viable cells to give an orange-coloured formazan dye.
Orangu™ demonstrates higher levels of sensitivity than commonly used alternatives MTT, XTT, MTS or WST-1.