Karyotype service for Mouse Cells
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Our mouse karyotype service provides G-banding analysis of a minimum of 20 metaphase spreads per sample, with a detailed report in ISCN nomenclature and at least one publication-quality image. Mouse karyotyping is essential quality control for mESC lines, transgenic models, and gene-edited cell lines. Common structural rearrangments include trisomy 8 and 11, as well as Robertsonian translocations in other species, such as pygmy, can affect phenotype and experimental reproducibility. We accept both fixed samples (BSL1 and BSL2, shipped at room temperature) and live cell cultures (BSL1, UK and Europe only). Standard turnaround is 7–10 business days. For a full overview of our karyotyping capabilities across all species, see our karyotype analysis service page
This service currently has good availability. Please contact us at [email protected] to schedule sample arrival.
Protocols and workflow
Biosafety level
We are able to process live BSL1 samples only. Fixed samples do not require evidence of screening.
Fixed cell requirements
Please strictly adhere to the following requirements for fixed samples:
- Samples are shipped at room temperature
- Fixed samples are stored in a 2 mL screw cap tube
- Samples are minimum 500 µL in volume
- Please do not send any samples without booking in advance
If you have any questions, please contact us by email at [email protected]. We will respond within one business day.
Product documents
Example of a normal mouse sample report.pdf Example of an abnormal mouse sample report.pdf
Citations
See the full updated list on Google Scholar
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Schöndorf DC, Elschami M, Schieck M, Ercan-Herbst E, Weber C, Riesinger Y, Kalman S, Steinemann D, and Ehrnhoefer DE. Generation of an induced pluripotent stem cell cohort suitable to investigate sporadic Alzheimer's Disease. (2018). Stem Cell Research Jan;34:101351.
Joshi PS, Modur V, Cheng J, Robinson K and Rao K. Characterization of immortalized human mammary epithelial cell line HMEC 2.6. (2017). Tumour biology Oct;39(10):1010428317724283.
Tidball AM, Dang LT, Glenn TW, Kilbane EG, Klarr DJ, Margolis JL, Uhler MD, and Parent JM. Rapid Generation of Human Genetic Loss-of-Function iPSC Lines by Simultaneous Reprogramming and Gene Editing. (2017). Stem Cell Reports 9(3): 725-731.
Ludtmann MHR, Arber C, Bartolome F, de Vicente M, Preza E, Carro E, Houlden H, Gandhi S, Wray S, and Abramov AY. Mutations in valosin-containing protein (VCP) decrease ADP/ATP translocation across the mitochondrial membrane and impair energy metabolism in human neurons. (2017). Journal of Biological Chemistry 292(21): 8907-8917.
Zhang Y, Schmid B, Nielsen TT, Nielsen JE, Clausen C, Hyttel P, Holst B, and Freude KK. Generation of a human induced pluripotent stem cell line via CRISPR-Cas9 mediated integration of a site-specific heterozygous mutation in CHMP2B. (2016). Stem Cell Research 17(1): 148-150.
Chen H, Aksoy I, Gonnot F, Osteil P, Aubry M, Hamela C, Rognard C, Hochard A, Voisin S, Fontaine E, et al. Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency. (2015). Nature Communications 6: 7095.
