Recombinant Mouse LIF

Code Description Price Qty
GFM200-5 Mouse LIF, 5 μg $50.00
GFM200-20 Mouse LIF, 20 μg $140.00
GFM200-100 Mouse LIF, 100 μg $300.00
GFM200-1000 Mouse LIF, 1 mg $1,640.00
Expression of the two common pluripotency markers Nanog and Oct-4 in the presence of mouse LIF was assessed with RT-PCR. Gapdh is the loading control.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human MCP-1 has a predicted Mw of 8.7 kDa.

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Description

Leukemia Inhibitory Factor (LIF) is a member of the interleukin-6 (IL-6) family that is made by a variety of adult and embryonic tissues. LIF signals through the glycoprotein 130 (gp130)/LIF receptor (LIFR) heterodimer to activate STAT3 and MAPK signaling. LIF functions during hematopoietic differentiation, neuronal cell differentiation, kidney development, and inflammatory processes. The application of mouse LIF to long-term culture systems promotes mouse embryonic stem cell (ESC) self-renewal and pluripotency, similar to the functional activity of FGF-2 in human ESC cell culture systems.

Activity Assay

Mouse LIF is fully biologically active when compared to standard. The specific activity of the sample is approximately 1 x 108 units/mg, in which 106 units are identical of 10 μg of pure protein sufficient to treat 1.0 L of stem cells including human embryonic stem cells, neural stem cells, hematopoietic stem cells, mesenchymal stem cells and induced pluripotent stem cells.

AA Sequence

MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA F

Alternative Names

Leukocyte Inhibitory Factor, leukemia inhibitory factor, cholinergic differentiation factor
Product Details
Length 181 aa
Molecular Weight 20 kDa
Structure Monomer
Source E. coli
Accession Number P09056
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM acetic acid at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.

Citations

Russell R, Ilg M, Lin Q, Wu G, Lechel A, Bergmann W, Eiseler T, Linta L, Kumar PP, Klingenstein M. A Dynamic Role of TBX3 in the Pluripotency Circuitry. (2015). Stem Cell Research 5(6)