Recombinant Human TGF-β1

Code Description Price Qty
GFH39-5 Recombinant Human TGF-β1, 5 μg £57.50
GFH39-20 Recombinant Human TGF-β1, 20 μg £207.50
GFH39-100 Recombinant Human TGF-β1, 100 μg £775.00
GFH39-100x10 Recombinant Human TGF-β1, 100 μg x 10 £2,550.00
GFH39-1000 Recombinant Human TGF-β1, 1 mg £2,550.00
Inhibition of IL-4-induced HT-2 cells proliferation assay for Human TGF-β1. Cell proliferation was measured to calculate the ED50, which is as expected less than 500 pg/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human TGF-β1 has a predicted Mw of 25.6 kDa (each monomer is 12.8 kDa).

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PODS Growth Factors

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Description

Transforming Growth Factor β 1 (TGF-β1) is a member of the TGF-β superfamily of cytokines. Members of this family exhibit regulatory activity in immunity, proliferation, adhesion, migration, and growth inhibition pathways. TGF-β1 signals through SMAD proteins via the TGF-β RI and TGF-β RII receptors.

Activity Assay

Human TGF-β1 is fully biologically active when compared to standard. The activity is determined by the ability to inhibit IL-4-induced HT-2 cells proliferation and it is typically less than 500 pg/ml. This corresponds to an expected specific activity of 2 x 106 units/mg.

AA Sequence

ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS

Alternative Names

Transforming Growth Factor β 1, TGF β 1, TGF-β-1, TGFB1, TGFβ1
Product Details
Length 112 / 224 aa
Molecular Weight 12.8 / 25.6 kDa
Structure Dimer
Source CHO cells
Accession Number P01137
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1 % trifluoroacetic acid (TFA) and Trehalose in a 20:1 Trehalose to protein ratio
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM HCl at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.