Recombinant Human Activin A
Guaranteed Quality
Purchase high quality, bioactive recombinant Human Activin A for your cell culture needs from Cell Guidance Systems.
PODS® Growth Factors
For sustained Activin A release in 3D culture or long-term differentiation protocols, this growth factor is also available as PODS® for sustained release, see here.
Description
Activin A is a critical component of directed differentiation protocols for iPSC and embryonic stem cell lines. It is most widely used for definitive endoderm induction, where it acts through Nodal/Activin signalling to drive mesendoderm and endoderm fate specification. Standard protocols combine Activin A with Wnt pathway activators (such as CHIR99021) over 3–5 days to generate CXCR4+/SOX17+ definitive endoderm cells, as a precursor to pancreatic, hepatic, and intestinal lineage differentiation. Activin A is also used to maintain pluripotency in human and mouse ESC culture, and as a TGF-beta superfamily ligand in studies of wound healing, reproductive biology, and haematopoiesis.
Activity Assay
Human Activin A is fully biologically active when compared to standard. The activity is determined using MPC-11 cells and it is typically 1.6 - 3.1 ng/ml. In addition to the standard assay, the activity of this product has been verified in the lab of Dr Josué Mfopou Kunjom (VUB - Diabetes Research Centre) - by analysis of CXCR4 in definitive endoderm.
AA Sequence
MGNICAKKQF FVSFKDIGWN DWIIAPSGYH ANYCEGECPS HIAGTSGSSL SFHSTVINHY RMRGHSPFAN LKSCCVPTKL RPMSMLYYDD GQNIIKKDIQ NMIVEECGCS
Alternative Names
Inhibin β-1, FRP, FSH-releasing protein, EDF, erythroid differentiation factor, FRP, follicle stimulating hormone releasing protein, Activin-A
Research Use Only
For professional research use only
| Product Details | |
|---|---|
| Length | 110 aa |
| Molecular Weight | 24.4 kDa |
| Structure | Dimer |
| Source | E. coli |
| Accession Number | P08476 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
