Recombinant Human BDNF
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PODS® Growth Factors
This growth factor is also available as PODS® for sustained release, see here.Description
Brain-derived neurotrophic factor (BDNF) is a nerve growth factor that binds two receptors, the low-affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase B (TrkB), to support neuron growth and survival. BDNF expression in the hippocampus is essential for long-term memory storage and learning. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (Neurotrophin-3). Human, mouse, rat, and pig BDNF are cross-reactive.Activity Assay
Human BDNF is fully biologically active when compared to standard. The activity is determined by the proliferation of C6 cells and it is typically less than 2 μg/ml. This corresponds to an expected specific activity of 5.0 x 102 units/mg.AA Sequence
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGRAlternative Names
Brain-derived neurotrophic factor, neurotrophin, abrineurin| Product Details | |
|---|---|
| Length |
120 aa |
| Molecular Weight |
27.2 kDa |
| Structure |
Monomer |
| Source |
E. coli |
| Accession Number |
P23560 |
| Purity |
=95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level |
=1.00 EU/®g as measured by kinetic LAL |
| Formulation |
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution |
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20®C to -80®C as supplied. 1 month when stored at 4®C after reconstituting as directed. 3 months when stored at -20®C to -80®C after reconstituting as directed. |
