PODS® universal cargo antibody (PUCA)
PODS® universal cargo antibody
Description
The PODS® universal cargo antibody (PUCA) binds to the hinge region between the H! affinity tag and the cargo molecules. It has been developed by Cell Guidance Systems to act as a universal antibody to detect and quantity cargo molecules released from PODS® crystals.
Product Usage Information
Western blotting: Dilution 1:5000
Storage
Supplied in PBS + 0.02% ProClin 300. Store at -20oC
Source
The monoclonal antibody was produced by immunizing animals with a synthetic peptide
corresponding to the H1 capture tag.
Specificity
The universal cargo antibody detects the H1 capture tag KNSRPSTSLYKKAGF
Product data
Lane 1 = Empty PODS
Lane 2 = PODS human SCF
Western blot analysis of empty and PODS human SCF
