EVlution™ Pro Automated TFF System
Product description
EVlution™ Pro is an automated benchtop tangential flow filtration (TFF) instrument that concentrates and diafilters up to 3 litres of biological fluid. It works with proteins, antibodies, nucleic acids, polysaccharides, EVs, viruses and VLPs.
Five Bluetooth sensors report feed and permeate mass, plus feed, retentate and permeate pressure. From these, EVlution Pro calculates TMP, flux, Lp, concentration factor and diavolumes in real time, and adjusts pump speed to hold the pressure you set. Set the target, start the run and leave it. Alarms and automatic shutdown protect the sample if conditions move outside defined limits.
Every run is stored against the cartridge that performed it and exported as an XLSX spreadsheet. The included analysis software for Mac and Windows compares permeability and recovery across runs, so you can see when a cartridge is due for retirement.
The EVlution™ Pro system includes:
- The EVlution Pro instrument with detachable touchscreen control console
- The EVlution pump
- Three Bluetooth pressure sensors (feed, retentate, permeate)
- Two Bluetooth balances (feed and permeate)
- A 94 cm2 or 260 cm2 cartridge, with ten pore sizes from 5 kDa to 1000 kDa
- Feed and permeate bottles, all tubing and valves
- EVlution Analysis software for Mac and Windows
Contact us for further information or a quotation at [email protected]
| Product Details | |
|---|---|
| Processable Volume |
Up to 1 litre with 94 cm2 cartridge, 3 litres with 260 cm2 cartridge |
| Final retentate volume |
Under 10 ml with SwitchFlow™ |
| Sensors |
5 Bluetooth: feed, retentate and permeate pressure; feed and permeate balances |
| Data export |
XLSX; Raw data, analysis software for Mac and Windows |
| Users |
Password-protected, multi-user |
| System weight and footprint |
Approx. 8 kg, 28 cm x 22 cm |
System overview
Sample type?
Why choose EVlution Pro?
Set it and walk away. Set the target, press start and leave the run to finish. As the retentate concentrates, the pump speeds up or slows down to hold the pressure you chose. If a reading moves out of range, an audible alarm sounds and the run shuts down before the sample is put at risk.
A complete record of every run. Five sensors report to the console throughout. TMP, flux, Lp and diavolumes are calculated live and saved with the run, which exports as an XLSX file. Nobody has to copy gauge readings into a lab book.
Sterile work in a flow cabinet. Lift the console off the instrument and stand it alongside. Everything carries on over Bluetooth, and the cabinet window can come down.
Small when stored. The balances and console stack into the main housing, taking up 28 x 22 cm at the side of the cabinet.
Down to under 10 ml. Once the automated concentration is done, SwitchFlow™ reverses the retentate flow to recover the sample in a final volume below 10 ml, usually in less than five minutes.
Cartridges you can keep track of. Cleaning cycles run at low pressure and stop by themselves. Each cartridge builds up its own history over as many as 20 runs, and the analysis software shows when its permeability has fallen far enough to retire it.
Gentle on fragile samples. Hollow fibre cartridges give low-shear, laminar flow, which helps keep EVs and enveloped viruses intact.
Built for shared labs. Each user signs in with their own password, which suits core facilities and multi-user groups.
Already using EVlution? EVlution Pro uses the same pump and the same hollow fibre cartridges as the manual EVlution TFF system.
Detailed monitoring and comprehensive analysis
Workflow. Run, filter, clean, calibrate and test are all programmed steps, logged against each run from a single activity screen.
Filtering. Feed, retentate and permeate pressures, pump speed, balance readings, flux and TMP are charted live, with elapsed time and an estimated finish. In auto pump mode, pump speed adjusts continuously to hold the target pressure.
Cleaning. Automated cycles at low, controlled pressure stop on their own and return each cartridge to service in a consistent state.
Analysis. Compare permeability and recovery across runs on the instrument or on a Mac or Windows computer. Lp trends against a retirement threshold show when a cartridge is due to be replaced.
Application Data
How tangential flow filtration works
Conventional dead-end filtration pushes the whole sample into the membrane, where retained material quickly builds up and slows filtration. TFF directs the flow across the membrane surface instead, sweeping it clean as it recirculates. This keeps permeate rates high and allows much larger volumes to be concentrated and diafiltered in one continuous run.
EVs
Ultracentrifugation becomes a bottleneck once EV work moves to conditioned media or biofluids above about 50 ml. Runs are long and sequential, and repeated high g-forces can damage vesicles and generate protein aggregates.
EVlution™ Pro processes 1 litre of conditioned medium to 20 ml of retentate in under 90 minutes, unattended. SwitchFlow™ then takes it below 10 ml in under five minutes. Free protein is removed during the same step, as shown by BCA assay, while NTA confirms EV recovery.
For high-purity preparations, follow TFF with Exo-spin™ SEC columns. The two steps together take you from large-volume conditioned media to material ready for proteomics, RNA analysis or functional assays.
Sample to EV workflow
EV concentration and removal of contaminating proteins
Data generated using the EVlution pump and cartridge, as used in EVlution Pro. 1000 ml of cancer cell conditioned media was processed at 1 bar in under 90 minutes to generate 20 ml of retentate. (A) Number of EV-sized particles determined by NTA. (B) Total protein in input, permeate and retentate measured by BCA assay.
Protein concentration
Data generated using the EVlution pump and cartridge, as used in EVlution Pro. Tenfold concentration of 500 ml of 1 mg/ml BSA to 50 ml. Each concentration was performed three times under the same conditions.
Hollow Fibre Filter Applications by Pore Size |
|||
| Pore Size (kDa) | Approx. Pore Diameter (nm) | Retained Molecules | Typical Applications |
| 5 | ~3 | Small proteins, nucleic acids, EVs | Protein concentration; removal of salts and small molecules |
| 8 | ~4 | Proteins, nucleic acids, EVs | Protein purification; diafiltration |
| 10 | ~5 | Proteins, cytokines, nucleic acids, EVs | Cytokine and growth factor concentration; buffer exchange |
| 30 | ~8 | Large proteins, antibody fragments, EVs | Antibody fragment purification; depletion of small proteins |
| 50 | ~12 | Albumin, large proteins, EVs | Albumin removal; large protein concentration |
| 100 | ~18 | Large protein complexes, EVs | Concentration of large protein complexes; pre-filtration for EV isolation |
| 300 | ~25 | EVs / Exosomes, large complexes | EV concentration and purification; FBS-EV depletion |
| 500 | ~45 | EVs / Exosomes, microvesicles | EV concentration and purification; FBS-EV depletion |
| 750 | ~65 | EVs, microvesicles, large particles | Microvesicle isolation; removal of smaller EVs and proteins |
| 1000 | ~100 | Large EVs, microvesicles, apoptotic bodies | Large particle concentration; apoptotic body isolation |
Most widely used pore sizes for EV concentration shown in bold
