Recombinant Human BAFF
Induced proliferation assay for Human BAFF. Cell proliferation was measured to calculate the ED50, which is as expected less than 30 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human BAFF has a predicted Mw of 18.5 kDa.
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DescriptionB cell Activating Factor (BAFF) is a type II member of the Tumor Necrosis Factor (TNF) superfamily. BAFF is expressed as a transmembrane protein on T cells, macrophages, and dendritic cells. The transmembrane domain of BAFF can also be cleaved to produce a soluble protein fragment. BAFF binds to the TNF receptors known as B cell maturation antigen (BCMA), transmembrane activator and CAml interactor (TACI), and BAFF receptor (BAFFR). BAFF is important for the survival and maturation of peripheral B cells. Human BAFF shows activity on mouse splenocytes.
Activity AssayHuman BAFF is fully biologically active when compared to standards. The ED50 is determined by a RPMI-8226 cell survival assay in the presence of 0.2 µM DEX and it is tipically less than 30 ng/ml. This corresponds to a specific activity of 6.7 x 104 units/mg.
AA SequenceMHHHHHHLVP RAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL
Alternative NamesB cell Activating Factor, TALL, BLyS, B lymphocyte stimulator, Tumor necrosis factor ligand superfamily member 13B, THANK
|Molecular Weight||18.5 kDa|
|Purity||≥90% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.