Recombinant Human BCA-1 / CXCL13
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DescriptionB cell-attracting chemokine 1 (BCA-1), also known as CXCL13, is expressed at high levels in lymphoid tissues, such as the spleen, lymph nodes, and Peyer's patches. BCA-1 activates signaling through the receptor Burkitt lymphoma receptor 1 (BLR1) to chemoattract B cells.
Activity AssayHuman BCA-1 is fully biologically active when compared to standards. The activity can be determined by its ability to chemoattract BaF3 cells transfected with CXCR5 and it is typically 5-20 ng/ml. This corresponds to the specific activity of 6.7 x 104 units/mg.
AA SequenceVLEVYYTSLR CRVQESSVFI PRRFIDRIQI LPRGNGCPRK EIIVWKKNKS IVCVDPQAEW IQRMMEVLRK RSSSTLPVPV FKRKIP
Alternative NamesB cell-attracting chemokine 1, B-cell-attracting chemokine 1, BCA1, BLC B cell-attracting chemokine 1, BLC, BLR1 Ligand, chemokine (C-X-C motif) ligand 13, CXCL13, small-inducible cytokine B13, small inducible cytokine B subfamily (Cys-X-Cys motif), CXC chemokine BLC, SCYB13, C-X-C motif chemokine 13, chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant), B-cell chemoattractant, B-lymphocyte chemoattractant, B lymphocyte chemoattractant, ANGIE, ANGIE2, B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1), BLR1L
|Molecular Weight||10.2 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.6|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.