Recombinant Human FGF-4 (Animal-Free)

Code Description Price Qty
GFH31AF-5 Human FGF-4 (Animal-Free), 5 μg $70.00
GFH31AF-25 Human FGF-4 (Animal-Free), 25 μg $180.00
GFH31AF-100 Human FGF-4 (Animal-Free), 100 μg $455.00
GFH31AF-1000 Human FGF-4 (Animal-Free), 1 mg $3,245.00
Induced proliferation of 3T3 cells assay for Human FGF-4 (Animal-Free). Cell proliferation was measured to calculate the ED50, which is as expected less than 1 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human FGF-4 has a predicted Mw of 19.4 kDa.

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PODS® Growth Factors

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Fibroblast Growth Factor 4 (FGF-4) is a secreted growth factor that is predominantly expressed during bone morphogenesis and embryonic limb development. FGF-4 is an important growth regulator for stem cells, fibroblasts, and endothelial cells. FGF-4 contains a single N-linked glycosylation signal. However, in vitro studies suggest that unglycosylated FGF-4 is cleaved into 13 kDa and 15 kDa truncated proteins that have greater biological activity than the wild type 19 kDa FGF-4 protein. Human FGF-4 shares high homology and is cross-reactive with mouse FGF-4.

Activity Assay

Human FGF-4 is fully biologically active when compared to standard. The activity is determined by the dose-dependent induced proliferation of NR6R-3T3 cells and it is typically less than 5 ng/ml. This corresponds to an expected specific activity of 2.0 x 105 units/mg.

AA Sequence

MAPTAPNGTL EAELERRWES LVALSLARLP VAAQPKEAAV QSGAGDYLLG IKRLRRLYCN VGIGFHLQAL PDGRIGGAHA DTRDSLLELS PVERGVVSIF GVASRFFVAM SSKGKLYGSP FFTDECTFKE ILLPNNYNAY ESYKYPGMFI ALSKNGKTKK GNRVSPTMKV THFLPRL

Alternative Names

Fibroblast Growth Factor 4, FGF4, FGF 4, transforming protein KS3, HBGF-4, HST-1
Product Details
Length 177 aa
Molecular Weight 19.4 kDa
Structure Monomer
Source E. coli
Accession Number P08620
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.