Recombinant Human G-CSF
Induced proliferation of NFS-60 cells assay for Human G-CSF. Cell proliferation was measured to calculate the ED50, which is as expected less than 60 pg/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human G-CSF has a predicted Mw of 18.8 kDa.
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PODS® Growth Factors
This growth factor is also available as PODS® for sustained release, see here.Description
Granulocyte-Colony Stimulating Factor (G-CSF) is a cytokine that functions as a potent inducer of neutrophilic granulocyte proliferation, terminal differentiation, and activation. G-CSF synthesis occurs in monocyte, macrophage, epithelial, endothelial, and fibroblast cells after activation by bacterial endotoxins, Tumor Necrosis Factor α (TNF-α), Interleukin-1 (IL-1), or Interleukin-17 (IL-17). The functional activity of G-CSF is mediated through the granulocyte colony-stimulating factor receptor (G-CSF-R) to activate JAK/STAT and MAPK signal transduction pathways. G-CSF also promotes neurogenesis and inhibits neuronal apoptosis. Human and mouse G-CSF proteins are cross-reactive.Activity Assay
Human G-CSF is fully biologically active when compared to standard. The activity is determined by the proliferation of NFS-60 cells and it is typically less than 60 pg/ml. This corresponds to an expected specific activity superior than 1.7 x 107 units/mg.AA Sequence
MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQPAlternative Names
Granulocyte Colony Stimulating Factor, granulocyte colony-stimulating factor, CSF-3, CSF3, MGI-1G, GM-CSF β, GM-CSFβ, pluripoietin, colony stimulating factor 3 (granulocyte), lenograstim, filgrastim, GCSF2, MGC45931, C17orf33, chromosome 17 open reading frame 33, CSF3OS, MGI-1G| Product Details | |
|---|---|
| Length | 175 aa |
| Molecular Weight | 18.8 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P09919 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 20 mM acetic acid, 50 mM sodium chloride |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
