Recombinant Human GDF-11 / BMP-11

Code Description Price Qty
GFH57-5 Human GDF-11, 5 μg $65.00
GFH57-20 Human GDF-11, 20 μg $165.00
GFH57-100 Human GDF-11, 100 μg $465.00
GFH57-1000 Human GDF-11, 1 mg $3,820.00
Induced alkaline phosphates activity in ATDC5 cells (with 1 μg of BMP-2 and 0.5% calf serum) for Human GDF-11. Alkaline phosphatase activity was measured to calculate the ED50.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human GDF-11 has a predicted Mw of 24.9 kDa (each monomer is 12.5 kDa).

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Description

Growth Differentiation Factor 11 (GDF-11), also known as BMP-11, is a regulator of cell growth and differentiation during muscular and neural development. GDF-11 binds the Transforming Growth Factor β receptors ALK4, ALK5, and ALK7 to activate SMAD signaling. In adults, exogenous GDF-11 promotes cardiomyocyte regeneration to reverse age-related cardiac hypertrophy.

Activity Assay

Human GDF-11 is fully biologically active when compared to standard. The activity is determined by the ability to decrease alkaline phosphatase activity in ATDC5 cells and it is typically less than 100 ng/ml. This corresponds to an expected specific activity of 1.0 x 104 units/mg.

AA Sequence

NLGLDCDEHS SESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGQCEYM FMQKYPHTHL VQQANPRGSA GPCCTPTKMS PINMLYFNDK QQIIYGKIPG MVVDRCGCS

Alternative Names

Growth Differentiation Factor 11, GDF11, GDF 11, Bone Morphogenic Protein 11, BMP11, BMP-11, BMP 11
Product Details
Length 109 / 218 aa
Molecular Weight 12.5 / 24.9 kDa
Structure Dimer
Source E. coli
Accession Number O95390
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM HCl at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.