Recombinant Human GM-CSF

Code Description Price Qty
GFH8-5 Human GM-CSF, 5 μg £55.00
GFH8-20 Human GM-CSF, 20 μg £130.00
GFH8-100 Human GM-CSF, 100 μg £330.00
GFH8-1000 Human GM-CSF, 1 mg £2,260.00
Induced proliferation of TF-1 cells assay for Human GM-CSF.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human GM-CSF has a predicted Mw of 14.6 kDa.

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PODS® Growth Factors

This growth factor is also available as PODS® for sustained release, see here.

Description

Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells. GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF promotes immune system development and regulates neutrophil function during infection. Human and mouse GM-CSF show no cross-reactivity.

Activity Assay

Human GM-CSF is fully biologically active when compared to standard. The activity is determined by a proliferation assay using TF-1 cells and it is typically less than 200 pg/ml. This corresponds to an expected specific activity of 5 x 106 units/mg.

AA Sequence

MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

Alternative Names

Granulocyte-Macrophage Colony-Stimulating Factor, GMCSF, CSF-2, MGI1GM, colony stimulating factor 2 (granulocyte-macrophage), colony-stimulating factor, CSF-2, MGIIGM, sargramostim, molgramostin
Product Details
Length 128 aa
Molecular Weight 14.6 kDa
Structure Monomer
Source E. coli
Accession Number P04141
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.

Citations

Lai HY, et al. CCAAT/enhancer-binding protein delta promotes intracellular lipid accumulation in M1 macrophages of vascular lesions. (2017). Cardiovascular Res 113(11): 1376-1388.