Recombinant Human GRO-γ / CXCL3
Induced chemotaxis of human neutrophils assay for Human GRO-γ.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human GRO-γ has a predicted Mw of 7.9 kDa.
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DescriptionGrowth regulated protein γ (GRO-γ), also called CXCL3, acts through the chemokine receptor CXCR2 to promote monocyte migration and adhesion. GRO-γ also induces the migration of cerebellar granule neuron precursor cells.
Activity AssayHuman GRO-γ is fully biologically active when compared to standard. The activity is determined by the induced chemotaxis of human neutrophils.
AA SequenceASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN
Alternative NamesGrowth regulated protein β, GROb, GROb1, CXCL2, MGSAβ, MGSA β, MGSA-b, CXCL2, MIP-2 α, MIP-2a, MIP2-α, MIP2A, MIP22, GRO2, chemokine (C-X-C motif) ligand 2, C-X-C motif chemokine 2, SCYB2, melanoma growth stimulatory activity β, CINC-2a, GROB3, GRO2 oncogene, macrophage inflammatory protein 2 α
|Molecular Weight||7.9 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)|
|Reconstitution||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Stability and Storage||
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.