Recombinant Human IL-33

Code Description Price Qty
GFH145-2 Human IL-33, 2 μg $65.00
GFH145-10 Human IL-33, 10 μg $165.00
GFH145-100 Human IL-33, 100 μg $635.00
GFH145-1000 Human IL-33, 1 mg $5,240.00
Induced proliferation of D10S cells assay for Human IL-33. Cell proliferation was measured to calculate the ED50, which is as expected less than 500 pg/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human IL-33 has a predicted Mw of 18.1 kDa.

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Description

Interleukin-33 (IL-33) is a member of the IL-1 cytokine family and is constitutively expressed in smooth muscle and airway epithelial cells. IL-33 signals through the interleukin-1 receptor-like 1 (IL-1R1) and interleukin-1 receptor accessory protein (IL1RAP) receptors to ativate NF-kappaB and MAPK signaling pathways. IL-33 functions to induce type 2 cytokine production in polarized Type 2 helper T (Th2) cells.

Activity Assay

Human IL-33 is fully biologically active when compared to standard. The activity is determined by the ability to induce D10S cells proliferation and it is typically less than 500 pg/ml. This corresponds to an expected specific activity of 2 x 106 units/mg.

AA Sequence

MSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET

Alternative Names

NP-HEV, interleukin-33, interleukin 33, IL33, IL 33
Product Details
Length 160 aa
Molecular Weight 18.1 kDa
Structure Monomer
Source E. coli
Accession Number O95760
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.