Recombinant Human PDGF-BB

Code Description Price Qty
GFH18-2 Human PDGF-BB, 2 μg $65.00
GFH18-10 Human PDGF-BB, 10 μg $165.00
GFH18-100 Human PDGF-BB, 100 μg $465.00
GFH18-1000 Human PDGF-BB, 1 mg $3,820.00
Induced profilferation of NIH 3T3 cells for Human PDGF-BB. Cell proliferation was measured to calculate the ED50, which is as expected less than 20 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human PDGF-BB has a predicted Mw of 24.9 kDa (each monomer is 12.4 kDa).

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PODS® Growth Factors

This growth factor is also available as PODS® for sustained release, see here.

Description

Platelet-Derived Growth Factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the α-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-a and PDGFR-b.

Activity Assay

Human PDGF-BB is fully biologically active when compared to standard. The activity is determined by the proliferation of NIH 3T3 cells and it is typically less than 20 ng/ml. This corresponds to an expected specific activity of 5.0 x 104 units/mg.

AA Sequence

MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT

Alternative Names

Platelet-Derived Growth Factor, GDGF, ODGF, PDGF BB
Product Details
Length 110 / 220 aa
Molecular Weight 12.4 / 24.9 kDa
Structure Dimer
Source E. coli
Accession Number P01127
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.