Recombinant Human Persephin (Animal-Free)

Code Description Price Qty
GFH81AF-5 Human Persephin (Animal-Free), 5 μg $70.00
GFH81AF-20 Human Persephin (Animal-Free), 20 μg $180.00
GFH81AF-100 Human Persephin (Animal-Free), 100 μg $455.00
GFH81AF-1000 Human Persephin (Animal-Free), 1 mg $3,245.00
Induced proliferation of TT cells assay for Human Persephin. Cell proliferation was measured to calculate the ED50, which is as expected less than 20 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human Persephin has a predicted Mw of 20.8 kDa (each monomer is 10.4 kDa).

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PODS® Growth Factors

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Persephin is a neurotrophic factor of the glial cell line-derived neurotrophic factor (GDNF) family. Persephin promotes survival and growth of dopaminergic and motor neurons, but not peripheral neurons. Persephin is a ligand for the RET receptor tyrosine kinase.

Activity Assay

Human Persephin (Animal-Free) is fully biologically active when compared to standard. The activity is determined by the ability to induce TT cells proliferation and it is typically less than 20 ng/ml. This corresponds to an expected specific activity of 4.0 x 104 units/mg.

AA Sequence

MALSGPCQLW SLTLSVAELG LGYASEEKVI FRYCAGSCPR GARTQHGLAL ARLQGQGRAH GGPCCRPTRY TDVAFLDDRHRWQRLPQLSA AACGCGG

Alternative Names

PSP
Product Details
Length 97 / 194 aa
Molecular Weight 10.4 / 20.8 kDa
Structure Dimer
Source E. coli
Accession Number O60542
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.