Recombinant Human TGF-β3 (Animal-Free)
Inhibition of IL-4 induced HT-2 cell proliferation for Human TGF-β3. Cell proliferation was measured to calculate the ED50, which is as expected less than 1 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human TGF-β3 has a predicted Mw of 25.7 kDa (each monomer is 12.9 kDa).
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PODS® Growth FactorsThis growth factor is also available as PODS® for sustained release, see here.
Animal-FreeThis product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
DescriptionTransforming Growth Factors (TGFs) are multifunctional peptides that regulate growth and differentiation in most cell types. The TGF-β family of proteins signal through serine/threonine kinase receptors. TGF-β isoforms (TGF-β1, -β 2, and -β 3) have overlapping, yet distinct biological actions in developing and adult tissues. TGF-β3 is an important factor in regulating cell adhesion and accelerating wound repair. TGF-β3 also functions during osteoblast proliferation, chemotaxis, and collagen synthesis.
Activity AssayHuman TGF-β3 is fully biologically active when compared to standard. The activity is determined by the inhibition of IL-4 induced HT-2 cell proliferation and it is typically less than 1 ng/ml. This corresponds to an expected specific activity of 1.0 x 106 units/mg.
AA SequenceMALDTNYCFR NLEENCCVRP LYIDFRQDLG WKWVHEPKGY YANFCSGPCP YLRSADTTHS TVLGLYNTLN PEASASPCCV PQDLEPLTIL YYVGRTPKVE QLSNMVVKSC KCS
Alternative NamesTransforming Growth Factor β 3, TGF β 3, TGF-β-3, TGFB3, TGFβ3
|Length||113 / 226 aa|
|Molecular Weight||12.9 / 25.7 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||In solution on 10 mM acetic acid and 20% ethanol at a concentration of 0.25 mg/ml|
|Stability and Storage||
12 months from date of receipt when stored at 4°C as supplied.