Recombinant Human TGF-β3
Inhibition of IL-4 induced HT-2 cell proliferation for Human TGF-β3. Cell proliferation was measured to calculate the ED50, which is as expected less than 1 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human TGF-β3 has a predicted Mw of 25.7 kDa (each monomer is 12.9 kDa).
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DescriptionTransforming Growth Factors (TGFs) are multifunctional peptides that regulate growth and differentiation in most cell types. The TGF-β family of proteins signal through serine/threonine kinase receptors. TGF-β isoforms (TGF-β1, -β 2, and -β 3) have overlapping, yet distinct biological actions in developing and adult tissues. TGF-β3 is an important factor in regulating cell adhesion and accelerating wound repair. TGF-β3 also functions during osteoblast proliferation, chemotaxis, and collagen synthesis.
Activity AssayHuman TGF-β3 is fully biologically active when compared to standard. The activity is determined by the inhibition of IL-4 induced HT-2 cell proliferation and it is typically less than 1 ng/ml. This corresponds to an expected specific activity of 1.0 x 106 units/mg.
AA SequenceMALDTNYCFR NLEENCCVRP LYIDFRQDLG WKWVHEPKGY YANFCSGPCP YLRSADTTHS TVLGLYNTLN PEASASPCCV PQDLEPLTIL YYVGRTPKVE QLSNMVVKSC KCS
Alternative NamesTransforming Growth Factor β 3, TGF β 3, TGF-β-3, TGFB3, TGFβ3
|Length||113 / 226 aa|
|Molecular Weight||12.9 / 25.7 kDa|
|Purity||≥95% determined by reducing and non-reducing SDS-PAGE|
|Endotoxin Level||≤1.00 EU/μg as measured by kinetic LAL|
|Formulation||In solution on 10 mM acetic acid and 20% ethanol at a concentration of 0.25 mg/ml|
|Stability and Storage||
12 months from date of receipt when stored at 4°C as supplied.