Recombinant Human TRAIL
Induced cytotoxicity of RPMI-8226 cells assay for Human TRAIL. Cell proliferation was measured to calculate the ED50.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human TRAIL has a predicted Mw of 19.5 kDa.
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Description
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the Tumor Necrosis Factor (TNF) family of cytokines. TRAIL is widely produced by a variety of cell types including tumor cells, smooth muscle of the lung and spleen, cerebellar glial cells, and thyroid follicular cells. TRAIL is a cytotoxic protein that induces apoptosis in tumor cells through the activation of the death receptors DR4 and DR5. TRAIL also binds the neutralizing decoy receptors, DcR1 and DcR2. Human TRAIL is active on mouse cells.Activity Assay
Human TRAIL is fully biologically active when compared to standard. The activity is determined by the ability to induce cytotoxicity to RPMI-8226 cells.AA Sequence
MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVGAlternative Names
Apo2L, TL2, Apo2 Ligand, TNFSF10, CD253| Product Details | |
|---|---|
| Length | 168 aa |
| Molecular Weight | 19.5 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P50591 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5 |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
