Recombinant Human TRAIL

Code Description Price Qty
GFH115-10 Human TRAIL, 10 μg $65.00
GFH115-50 Human TRAIL, 50 μg $165.00
GFH115-100 Human TRAIL, 100 μg $295.00
GFH115-1000 Human TRAIL, 1 mg $1,310.00
Induced cytotoxicity of RPMI-8226 cells assay for Human TRAIL. Cell proliferation was measured to calculate the ED50.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human TRAIL has a predicted Mw of 19.5 kDa.

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Description

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the Tumor Necrosis Factor (TNF) family of cytokines. TRAIL is widely produced by a variety of cell types including tumor cells, smooth muscle of the lung and spleen, cerebellar glial cells, and thyroid follicular cells. TRAIL is a cytotoxic protein that induces apoptosis in tumor cells through the activation of the death receptors DR4 and DR5. TRAIL also binds the neutralizing decoy receptors, DcR1 and DcR2. Human TRAIL is active on mouse cells.

Activity Assay

Human TRAIL is fully biologically active when compared to standard. The activity is determined by the ability to induce cytotoxicity to RPMI-8226 cells.

AA Sequence

MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG

Alternative Names

Apo2L, TL2, Apo2 Ligand, TNFSF10, CD253
Product Details
Length 168 aa
Molecular Weight 19.5 kDa
Structure Monomer
Source E. coli
Accession Number P50591
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.