Recombinant Mouse G-CSF

Code Description Price Qty
GFM14-2 Recombinant Mouse G-CSF, 2 μg £50.00
GFM14-10 Recombinant Mouse G-CSF, 10 μg £120.00
GFM14-100 Recombinant Mouse G-CSF, 100 μg £375.00
GFM14-100x10 Recombinant Mouse G-CSF, 100 μg x 10 £1,800.00
GFM14-1000 Recombinant Mouse G-CSF, 1 mg £1,800.00
Induced proliferation of NFS-60 cells assay for Mouse G-CSF. Cell proliferation was measured to calculate the ED50, which is as expected less than 50 pg/ml.

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Description

Granulocyte-Colony Stimulating Factor (G-CSF) is a cytokine that functions as a potent inducer of neutrophilic granulocyte proliferation, terminal differentiation, and activation. G-CSF synthesis occurs in monocyte, macrophage, epithelial, endothelial, and fibroblast cells after activation by bacterial endotoxins, Tumor Necrosis Factor α (TNF-α), Interleukin-1 (IL-1), or Interleukin-17 (IL-17). The functional activity of G-CSF is mediated through the granulocyte colony-stimulating factor receptor (G-CSF-R) to activate JAK/STAT and MAPK signal transduction pathways. G-CSF also promotes neurogenesis and inhibits neuronal apoptosis. Human and mouse G-CSF proteins are cross-reactive.

Activity Assay

Mouse G-CSF is fully biologically active when compared to standard. The activity is determined by the proliferation of NFS-60 cells and it is typically less than 50 pg/ml. This corresponds to an expected specific activity of 2 x 107 units/mg.

AA Sequence

MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA

Alternative Names

Granulocyte Colony Stimulating Factor, granulocyte colony-stimulating factor, CSF-3, CSF3, MGI-1G, GM-CSF β, GM-CSFβ, pluripoietin, colony stimulating factor 3 (granulocyte), lenograstim, filgrastim, GCSF2, MGC45931, C17orf33, chromosome 17 open reading frame 33, CSF3OS, MGI-1G
Product Details
Length 179 aa
Molecular Weight 19.1 kDa
Structure Monomer
Source E. coli
Accession Number P09920
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium citrate, pH 3.0
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.