Recombinant Mouse SCF (Animal-Free)

Code Description Price Qty
GFM9AF-10 Mouse SCF (Animal-Free), 10 μg $206.00
GFM9AF-100 Mouse SCF (Animal-Free), 100 μg $522.00
GFM9AF-500 Mouse SCF (Animal-Free), 500 μg $1,744.00
GFM9AF-1000 Mouse SCF (Animal-Free), 1 mg $2,895.00
Induced proliferation of TF-1 cells assay for Mouse SCF. Cell proliferation was measured to calculate the ED50, which is as expected less than 20 ng/ml.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Mouse SCF has a predicted Mw of 18.4 kDa.

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PODS® Growth Factors

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor c-Kit/CD117 and plays a critical role in the maintenance, survival, and differentiation of hematopoietic stem cells. Human SCF shows no activity on mouse cells, but mouse and rat SCF are active on human cells.

Activity Assay

Mouse SCF is fully biologically active when compared to standard. The activity is determined by the dose-dependent proliferation of TF-1 cells and it is typically less than 20 ng/ml. This corresponds to an expected specific activity of 5 x 104 units/mg.

AA Sequence

MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA

Alternative Names

Stem Cell Factor, c-Kit Ligand, KL, steel factor, MGF
Product Details
Length 165 aa
Molecular Weight 18.6 kDa
Structure Monomer
Source E. coli
Accession Number P21583
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 20 mM sodium phosphate, pH 6.5
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.