Recombinant Rat GDNF (Animal-Free)

Code Description Price Qty
GFR10AF-10 Rat GDNF (Animal-Free), 10 μg $206.00
GFR10AF-100 Rat GDNF (Animal-Free), 100 μg $797.00
GFR10AF-500 Rat GDNF (Animal-Free), 500 μg $3,889.00
GFR10AF-1000 Rat GDNF (Animal-Free), 1 mg $6,609.00
Induced proliferation of C6 cells assay for Rat GDNF. Cell proliferation was measured to calculate the ED50.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Rat GDNF has a predicted Mw of 30.1 kDa (each monomer is 15.1 kDa).

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PODS® Growth Factors

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Animal-Free

This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.

Description

Glial cell-derived neurotrophic factor (GDNF) is a neurotrophic factor that signals through a multicomponent receptor system to activate receptor tyrosine kinase RET signaling. GDNF promotes dopamine uptake, prevents motor neuron apoptosis, and supports the survival and differentiation of neurons.

Activity Assay

Rat GDNF is fully biologically active when compared to standard. The activity is determined by a proliferation assay using C6 cells.

AA Sequence

MSPDKQAAL PRRENRNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Alternative Names

ATF, HFB1-GDNF, ATF1, ATF-1, astrocyte-derived trophic factor 2, glial cell line derived neurotrophic factor
Product Details
Length 135 / 270 aa
Molecular Weight 15.1 / 30.2 kDa
Structure Dimer
Source E. coli
Accession Number P48540
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.