Recombinant Rat IL-1 β
Induced proliferation of D10S cells assay for Rat IL-1 β. Cell proliferation was measured to calculate the ED50.
Achieve Purchasing Synergy
Request a quote at [email protected] for 2 or more vials of any Growth Factor to receive a discount. Discounts range from 5 to 20% according to the size of your order.
PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Description
Interleukin-1 β (IL-1 β) is a pro-inflammatory cytokine that is produced by monocytes, macrophages, and dendritic cells (DCs). IL-1 β signals through the IL-1 receptor, type 1 (IL-1R1) to activate the myeloid differentiation primary response 88 (MYD88) signaling pathway, which contains the cytoplasmic Toll/IL-1 receptor (TIR) domain adapter. IL-1 β and the independently regulated IL-1 α protein have overlapping proinflammatory activities to induce adhesion molecule expression on epithelial cells, control fever induction, initiate rheumatoid arthritis, and promote septic shock.Activity Assay
Rat IL-1 β is fully biologically active when compared to standard. The activity is determined by the ability to induce D10S cells proliferation.AA Sequence
MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSSAlternative Names
Interleukin-1 β, preinterleukin 1 β, IL1, IL 1, catabolin, LAF, EP, LEM, MCF| Product Details | |
|---|---|
| Length | 153 aa |
| Molecular Weight | 17.4 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | Q63264 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5 |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
