Recombinant Rat IL-10
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Rat IL-10 has a predicted Mw of 18.8 kDa.
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Description
Interleukin-10 (IL-10) is an anti-inflammatory cytokine produced by macrophages and type 2 T helper (Th2) cells. IL-10 inhibits the production of pro-inflammatory cytokines such as interferon-γ (IFN-γ), Tumor Necrosis Factor α (TNF-α), interleukin-2 (IL-2), interleukin-3 (IL-3), interleukin-4 (IL-4), and Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), made by macrophages and regulatory T cells. IL-10 also suppresses antigen presentation on antigen presenting cells, and enhances the survival, proliferation, and antibody production of B cells.Activity Assay
Activity to be determined.AA Sequence
MSKGHSIRGD NNCTHFPVSQ THMLRELRAA FSQVKTFFQK KDQLDNILLT DSLLQDFKGY LGCQALSEMI KFYLVEVMPQ AENHGPEIKE HLNSLGEKLK TLWIQLRRCH RFLPCENKSK AVEQVKNDFN KLQDKGVYKA MNEFDIFINC IEAYVTLKMK NAlternative Names
Interleukin-10, interleukin 10, IL10, IL 10, B-TCGF, CSIF, TGIF, cytokine synthesis inhibitory factor| Product Details | |
|---|---|
| Length | 161 aa |
| Molecular Weight | 18.8 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P29456 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
