RGD (Arg-Gly-Asp) peptide, 5 mg

Research Use Only

Procedures

Please note: Use these recommendations as guidelines to determine the optimal coating conditions for your cultures system. Two options are provided: procedure A and procedure B.

Procedure A

1. Remove cap and add 5 ml of serum-free medium or PBS to the bottle. 
2. Replace cap and vortex contents vigorously. Ensure that the RGD peptide is completely solubilized. The solution will remain slightly hazy.
3. Transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using serum-free medium or PBS. A typical working concentration may range from 0.1 to 10 μg/ml.
4. Sterile filter solution through a 0.22 micron button filter.
5. Aseptically add appropriate amount of diluted, sterile material to culture surface.
6. Incubate at room temperature or 37°C, covered, for 1 - 2 hours.
7. After incubation, aspirate remaining material.
8. Rinse plates carefully with dH2O - avoid scratching bottom surface of plates.
9. Plates are ready for use. They may also be stored at 2 - 10°C damp or air dried if sterility is maintained.
10. Store remaining solubilized RGD peptide at 2 - 10°C. Additional note: Include divalent cations (Calcium, Magnesium, or Manganese) in cell attachment solution to obtain optimum cell binding.

Procedure B

1. Remove cap and add 5 ml of sterile 70% ethanol.
2. Replace cap and vortex contents. Ensure that the RGD peptide is completely solubilized.
3. Transfer desired volume of solution from the bottle to a dilution vessel. Dilute to the desired concentration using 70% ethanol. Concentrations from 0.1 to 10 μg/ml should be tested.
4. Add appropriate amount of diluted material to culture surface.
5. Leave the coated container, uncovered, in a laminar flow hood until the wells are dry.
6. Rinse plates carefully with dH2O - avoid scratching bottom surface of plates.
7. Plates are ready for use.
8. Store remaining solubilized RGD peptide at 2 - 10°C.