Poly-D-Lysine (0.1 mg/ml), 5 mg

Code Description Price Qty
AP17 Poly-D-Lysine (0.1 mg/ml), 5 mg £85.00

Description

Poly-D-Lysine is a synthetic amino acid chain that is positively charged. The molecular weight of Poly-D-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product's molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Applications

Poly-D-Lysine enhances cell binding to polystyrene surfaces for certain cell-based assays and procedures.
Product Details
Source Synthetic
Molecular Weight 70 - 150 kDa
pH 7.0 - 7.6
Sterilization Method Irradiation
Sterility No growth
Cell Attachment Assay Pass
Concentration 0.1 mg/ml
Storage and Stability 4°C

Protocols

Instructions for Use

Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system. To maintain sterility, perform all operations in a laminar flow hood.

1. A typical working concentration is 0.1 mg/ml. If a different concentration is desired, transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using tissue culture grade water or PBS.

2. Add appropriate amount of diluted material to culture surface. Typically, 1 ml per 25 cm2 is used. Rock gently to ensure uniform coating of culture surface.

3. After 5 minutes, remove excess solution by aspiration.

4. Thoroughly rinse surface with tissue culture grade water.

5. Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours.

6. Introduce medium and cells to the culture surface.

7. Store remaining Poly-D-Lysine at 2 - 10°C.

Documents

AP17 Datasheet.pdf