EVlution TFF System new
Product description
EVlution™ is a compact benchtop tangential flow filtration (TFF) instrument that provides efficient size-dependent concentration and purification of a range of biomolecules, including nucleic acids, proteins, viruses and extracellular vesicles (EVs).
Developed and supported by scientists with decades of experience in separation technology, EVlution™ TFF provides an effective method of size-based separation of biomolecules, whilst minimising membrane fouling. The innovative characteristics of EVlution™ design make it an excellent choice for workflows at the lab scale, supporting a wide range of hollow fiber filter configurations. EVlution™ simplifies complex filtration processes while delivering reliable performance.
The EVlutionTM System includes everything required to perform TFF isolation:
- The EVlution stand
- The EVlution pump
- A 94cm2 or 260m2 cartridge with pore sizes 5 kDa to 1000 kDa
- A pressure gauge (syringe-based disposable or digital options)
- All tubing and valves
THe EVlutionTM system's non-disposable parts are backed by a 2 year warranty.
Contact us now for further information or to place your order [email protected]
| Product Details | |
|---|---|
| Storage temperature |
Room temperature |
| Processable Volume |
Up to 1 litre with 94 cm2 cartridge, 3 litres with 260 cm2 cartridge |
| Supplied cartridge area |
94 cm2 and 260 cm2 cartidges are available |
| Cartridge pore size |
10 pore sizes available from 5 kDa to 1000 kDa |
| Pumping Capacity |
0.1 - 86 ml/min |
| System weight |
2.7 kg |
| Footprint |
21 cm x 26.5 cm |
What's your sample?
Video
Why choose EVlution?
Hollow fibre technology. EVlution uses hollow fibre filter cartridges to provide the low-shear, laminar flow environment that extracellular vesicles need. This preserves vesicle integrity and biological function, giving you confidence that what you isolate faithfully represents what was in your sample.
Innovative Switch Flow™ for the smallest output volumes. The ability to switch from circulated to reversible retentate flow enables a final retentate volume below 10 ml
Fully displaceable sample contact surfaces. By incorporating a disposable syringe-based pressure gauge, we have made all surfaces that the sample flows through disposable, allowing easy maintenance.
Genuinely affordable. EVlution is accessible to research groups, core facilities and teaching laboratories.
Compact benchtop footprint. With dimensions of just 21 × 26.5 cm and a weight of 2.7 kg, EVlution takes up less bench space than a standard laboratory balance. It requires no dedicated infrastructure - just plug in and go.
Flexible filter configurations. The adaptable design supports a range of hollow fibre cartridges from 5 kDa or 1000 kDa MWCO options, along with different cartridge sizes (94 cm² and 260 cm² membrane area). This allows you to match the system to your specific application, up to 3 litres.
Simple, intuitive operation. EVlution has been designed for ease of use. Researchers can set up and run a TFF process without extensive training or bioprocessing expertise. The streamlined workflow lowers the barrier to adoption and reduces the risk of operator error.
Proven for protein purification. EVlution efficiently concentrates proteins from complex samples, filtering out smaller molecules and metabolites.
Proven for EV processing. EVlution significantly reduces the concentration of free proteins in the retentate during EV concentration, as shown by BCA protein assay, while NTA particle tracking confirms efficient recovery of EV particles. This dual evidence of concentration and purification in a single step saves time and reduces sample handling.
Key Features
A capable TFF for EV work
As EV research scales up — from pilot experiments to functional studies, preclinical work, or process development — ultracentrifugation quickly becomes the bottleneck. It processes small volumes at a time, requires long sequential runs, and the repeated high gravitational forces can damage vesicle structure and introduce protein aggregates that compromise downstream analysis. For labs regularly working with conditioned media or biofluids at volumes above 50 ml, it simply doesn't hold up.
Tangential flow filtration takes a fundamentally different approach. Rather than forcing the entire sample through a membrane, TFF recirculates the sample parallel to the filter surface, continuously sweeping it clean and preventing fouling. This makes it possible to concentrate and diafilter large volumes in a single continuous run — removing free proteins and small molecules while keeping vesicles intact and in suspension throughout.
EVlution™ processes up to 1 litre of starting material in 90 minutes, generating a concentrated retentate of less than 10 ml via the Switch Flow™ system. Protein removal is confirmed by BCA assay, and EV recovery is tracked by NTA particle analysis — giving researchers measurable confidence in both purity and yield from each run.
EVlution™ is designed to integrate directly into a two-step workflow: TFF concentration and initial purification, followed by Exo-spin™ SEC columns for high-purity polishing. Together, the two systems cover the full range from large-volume conditioned media processing down to the final clean preparation needed for proteomics, RNA analysis, or functional assays.
Sample to EV workflow
EV concentration and removal of contaminating proteins with EVlution
EVlution TFF significantly reduces the concentration of free proteins within a sample when concentrating cell culture media. 1000 mL of cancer cell conditioned media was processed at 1 bar in under 90 minutes to generate 20 ml of retentate. (A) Number of EV-sized particles determined by NTA. (b) Total amount of protein in input, permeate and retentate measured using a BCA assay.
Use EVlution with CellGS hollow fibre cartridges for protein purification
10 x Concentration of a 500 ml of a 1mg/ml BSA sample to 50 ml. Each concentration was performed three times under the same conditions
Video
Setting up and using EVlution
Hollow Fibre Filter Applications by Pore Size |
|||
| Pore Size (kDa) | Approx. Pore Diameter (nm) | Retained Molecules | Typical Applications |
| 5 | ~3 | Small proteins, nucleic acids, EVs | Protein concentration; removal of salts and small molecules |
| 8 | ~4 | Proteins, nucleic acids, EVs | Protein purification; diafiltration |
| 10 | ~5 | Proteins, cytokines, nucleic acids, EVs | Cytokine and growth factor concentration; buffer exchange |
| 30 | ~8 | Large proteins, antibody fragments, EVs | Antibody fragment purification; depletion of small proteins |
| 50 | ~12 | Albumin, large proteins, EVs | Albumin removal; large protein concentration |
| 100 | ~18 | Large protein complexes, EVs | Concentration of large protein complexes; pre-filtration for EV isolation |
| 300 | ~25 | EVs / Exosomes, large complexes | EV concentration and purification; FBS-EV depletion |
| 500 | ~45 | EVs / Exosomes, microvesicles | EV concentration and purification; FBS-EV depletion |
| 750 | ~65 | EVs, microvesicles, large particles | Microvesicle isolation; removal of smaller EVs and proteins |
| 1000 | ~100 | Large EVs, microvesicles, apoptotic bodies | Large particle concentration; apoptotic body isolation |
Most widely used pore sizes for EV concentration
