Recombinant Human MIF
Induced IL-8 production by human PBMC cells when cultured with Human MIF.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human MIF has a predicted Mw of 12.5 kDa.
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PODS® Growth Factors
The product is currently not available as PODS®, please check back in the future.Description
Migration Inhibitory Factor (MIF) is a pro-inflammatory lymphokine that functions during cell-mediated immmunity. MIF promotes fibroblast migration by inducing interleukin-1 (IL-1), interleukin-8 (IL-8), and matrix metalloproteinase (MMP) expression. In interferon-γ-activated macrophages, MIF stimulates nitric oxide (NO) production and Tumor Necrosis Factor α (TNF-α) secretion.Activity Assay
Human MIF is fully biologically active when compared to standard. The activity is determined by the ability to produce IL-8 by human PBMC cells.AA Sequence
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFAAlternative Names
Migration Inhibitory Factor, GIF, phenylpyruvate tautomerase, glycosylation-inhibiting factor, L-dopachrome tautomerase| Product Details | |
|---|---|
| Length | 115 aa |
| Molecular Weight | 12.5 kDa |
| Structure | Monomer |
| Source | E. coli |
| Accession Number | P14174 |
| Purity | ≥95% determined by reducing and non-reducing SDS-PAGE |
| Endotoxin Level | ≤1.00 EU/μg as measured by kinetic LAL |
| Formulation | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Reconstitution | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| Stability and Storage |
12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed. |
Citations
Varinelli L, Caccia D, Volpi CC, Caccia C, De Bortoli M, Taverna E, Gualeni AV, Leoni V, Gloghini A, Manenti G, et al. 4-IPP, a selective MIF inhibitor, causes mitotic catastrophe in thyroid carcinomas. (2015).
Documents
GFH153 Datasheet.pdfMSDS Recombinant Proteins.pdf
