Recombinant Human MIF

Code Description Price Qty
GFH153-5 Human MIF, 5 μg $65.00
GFH153-25 Human MIF, 25 μg $165.00
GFH153-100 Human MIF, 100 μg $410.00
GFH153-1000 Human MIF, 1 mg $2,950.00
Induced IL-8 production by human PBMC cells when cultured with Human MIF.
Non-reducing (-) and reducing (+) conditions in a 4 - 20% Tris-Glycine gel stained with Coomassie Blue. 1 μg of protein was loaded in each lane. Human MIF has a predicted Mw of 12.5 kDa.

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Description

Migration Inhibitory Factor (MIF) is a pro-inflammatory lymphokine that functions during cell-mediated immmunity. MIF promotes fibroblast migration by inducing interleukin-1 (IL-1), interleukin-8 (IL-8), and matrix metalloproteinase (MMP) expression. In interferon-γ-activated macrophages, MIF stimulates nitric oxide (NO) production and Tumor Necrosis Factor α (TNF-α) secretion.

Activity Assay

Human MIF is fully biologically active when compared to standard. The activity is determined by the ability to produce IL-8 by human PBMC cells.

AA Sequence

MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

Alternative Names

Migration Inhibitory Factor, GIF, phenylpyruvate tautomerase, glycosylation-inhibiting factor, L-dopachrome tautomerase
Product Details
Length 115 aa
Molecular Weight 12.5 kDa
Structure Monomer
Source E. coli
Accession Number P14174
Purity ≥95% determined by reducing and non-reducing SDS-PAGE
Endotoxin Level ≤1.00 EU/μg as measured by kinetic LAL
Formulation Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Stability and Storage

12 months from date of receipt when stored at -20°C to -80°C as supplied. 1 month when stored at 4°C after reconstituting as directed. 3 months when stored at -20°C to -80°C after reconstituting as directed.

Citations

Varinelli L, Caccia D, Volpi CC, Caccia C, De Bortoli M, Taverna E, Gualeni AV, Leoni V, Gloghini A, Manenti G, et al. 4-IPP, a selective MIF inhibitor, causes mitotic catastrophe in thyroid carcinomas. (2015).

Documents

GFH153 Datasheet.pdfMSDS Recombinant Proteins.pdf