Exo-spin™ midi columns

Code Description Price Qty
EX04-5 Exo-spin™, 5 midi columns £125.00
EX04-20 Exo-spin™, 20 midi columns £355.00
EX06-30 Exo-spin™ buffer, 30 ml £35.00
EX06-250 Exo-spin™ buffer, 250 ml £160.00
Exo-spin™ midi column
Exo-spin™ midi columns

Product description

Exo-spin™ products provide a proven, rapid and reliable way of generating high quality purified exosomes suitable for a range of research applications.

Exo-spin™ technology is based on size exclusion chromatography (SEC), which is reliable for exosome isolation. A concentration step is recommended if a starting sample has low exosome-content. This includes cell culture media, urine, saliva etc. Without this important step, a lower exosome recovery will be obtained. Exo-spin™ provides a simple protocol which allows you to purify your sample in less than 2 hours with consistent and reliable results.

 

The EX04 Exo-spin™ product contains packed and equilibrated ready-to-use SEC columns.

The EX04 Exo-spin™ kit has been developed to process up to 500 ml of cell culture medium, saliva, urine (when used in conjunction with a conncentrating (e.g. precipitation) step. 1 ml of blood samples (plasma and sera) may be added onto the column directly.

Products on this page contain Exo-spin™ midi columns

 

Which Exo-spin™ kit shall I choose for my sample volume?

 

 

Low exosome-content biofluids (up to 1x109/ml)

including cell culture media, saliva, cerebrospinal fluid, urine

Sample Volume

isolation method

Exo-spin Kit

column bed length

< 1ml to 50 ml 

concentration + SEC

EX01 mini

1.3 cm 

 < 1 ml to 75 ml Note 1

concentration + SEC

EX05 mini-HD

6.35 cm

75 ml to 500 ml Note 1 concentration + SEC EX04 midi 2.15 cm

 

Plasma and Sera

Sample Volume

isolation method

Exo-spin Kit

column bed length

< 100 µl Note 2 

SEC

EX03 mini columns

1.3 cm 

< 150 µl 

SEC

EX05  mini-HD 6.35 cm 
100 µl - 250 µl Note 3 concentration + SEC EX02 mini blood 1.3 cm  
1 ml SEC EX04 midi 2.15 cm 

 

Note 1:  For cerebrospinal fluid (CSF) and human breast milk samples, validated protocols are available for EX01 only. The protocols are provided in the user guides. Note 2: Sera only. Note 3: Up to 500 ul for sera. 

Exo-spin™ column and resin pore size

The column bed volume in EX04 Exo-spin™ midi-columns is 10 ml, allowing for 1 ml of volume to be loaded onto each column.

The pore size within the resin is approximatively 30 nm to attain a highly pure exosome elution. All other molecules (e.g. proteins, lipids) which are smaller than 30 nm will enter into the pores and remain trapped in the column. 

Highest recovery and purity

Exosomes in a range 30-250 nm will elute in the first fraction. All other proteins and lipids will be retained in the column and will elute later than the exosomes, ensuring a highly pure sample ready for downstream application.

Reproducibility

All our columns are manufactured in our laboratory to ensure a high reproducibility between each lot. As proof of reproducibility and batch-to-batch consistency, a large number of peer-reviewed scientific papers have been published describing the use of Exo-spin™.

Storage

Upon receipt, store purification columns and Exo-spin™ Buffer at 4°C. All other components should be stored at room temperature (15°C - 25°C).

Frequently Asked Questions (FAQs)

For any additional questions, please refer to FAQs document below.

If you are just starting to work with exosomes, consider our starter pack

We designed the ideal starter pack to guide your exosome research. The starter pack includes the exosome purification kit of your choice, exosome validated antibodies, and NTA profiling analysis. Complete details can be found in the product page here.

 

Product data

EX04 Exo-spin™ midi columns comparative data

SEC Columns, Exosome yield

Comparison of total exosome recovery from 10 ml conditioned cell culture medium. The total particle yield obtained with Exo-spin™ buffer + Exo-spin™ midi columns is greater than the yield obtained using the membrane affinity method. Notably, Exo-spin™ buffer is much more efficient than generic PEG 6000 when used prior to Exo-spin™ midi column exosome isolation.
Isolated exosomes, NTA data
Data determined by nanoparticle tracking analysis (NTA). Each curve represents the average of 3 technical replicate measurements for each exosome isolation method triplicate experiment. (PM = Precipitation Method). Figure taken and adapted from (Martins, TS et al., 2018).

If you would like more information on the exosome extraction profile using Exo-spin™ midi columns, please refer to the EX04 technical note below in the documents section.

 

Characterizing Exo-spin™ isolated exosomes using nanoparticle tracking analysis (NTA)

Nanoparticle Tracking Analysis NTA

Exosome characterization with NTA. Exosomes have been isolated using the Exo-spin™ kit and analysis performed with the ZetaView® instrument.

Downstream applications: advice and content

The EX04 Exo-spin™ kit is compatible with all downstream application and has been published in a large range of different applications.

RNA analysis:

  • Santangelo L, Giurato G, Cicchini C, Montaldo C, Mancone C, Tarallo R, Battistelli C, Alonzi T, Weisz A and Tripodi M. (2016). The RNA-Binding Protein SYNCRIP Is a Component of the Hepatocyte Exosomal Machinery Controlling MicroRNA Sorting. Cell Reports 17(3):799-808.
  • Brook AC, Jenkins RH, Clayton A, Kift-Morgan A, Baby AC, Shephard AP, Mariotti B, Cuff SM, Bazzoni F, Bowen T, Fraser DJ, and Eberl M. (2019). Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis. Scientific Reports 9(1):10136. 

Immunoassay:

  • Salimu J, Webber J, Gurney M, Al-Taei S, Clayton A and Tabi Z. (2017). Dominant immunosuppression of dendritic cell function by prostate-cancer-derived exosomes. Journal of Extracellular Vesicles 6(1):1368823.

Protein assay:

  • Welton JL, Webber JP, Botos LA, Jones M and Clayton A. (2015). Ready-made chromatography columns for extracellular vesicle isolation from plasma. Journal of Extracellular Vesicles 4:27269.

Application note:

Citations

Brook AC, Jenkins RH, Clayton A, Kift-Morgan A, Baby AC, Shephard AP, Mariotti B, Cuff SM, Bazzoni F, Bowen T, Fraser DJ, and Eberl M. (2019). Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis. Scientific Reports 9(1):10136. 

Salimu J, Webber J, Gurney M, Al-Taei S, Clayton A and Tabi Z. (2017). Dominant immunosuppression of dendritic cell function by prostate-cancer-derived exosomes. Journal of Extracellular Vesicles 6(1):1368823.

Santangelo L, Giurato G, Cicchini C, Montaldo C, Mancone C, Tarallo R, Battistelli C, Alonzi T, Weisz A and Tripodi M. (2016). The RNA-Binding Protein SYNCRIP Is a Component of the Hepatocyte Exosomal Machinery Controlling MicroRNA Sorting. Cell Reports 17(3):799-808.

Welton JL, Webber JP, Botos LA, Jones M and Clayton A. (2015). Ready-made chromatography columns for extracellular vesicle isolation from plasma. Journal of Extracellular Vesicles 4:27269.