OptiCol™ Human Collagen Type I (lyophilized), 15 mg

Code Description Price Qty
M11L Human Collagen Type I (lyophilized), 15 mg £255.00

Description

OptiCol™ Human Collagen Type I is isolated from human neo-natal fibroblast cells. These cells are cultured in optimal conditions allowing these cells to naturally and efficiently secrete extracellular matrix which is processed and purified to produce human collagen. Manufactured under stringent quality standards with high lot-to-lot consistency. Made up of 97% Type I human collagen and 3% Type III collagen. OptiCol™ Human Collagen Type I exhibits high monomer content (as measured by gel permeation chromatography).

Applications

Tissue engineering research | Wound healing constructs | Coatings | Solid free-form fabrications | Electrospinning | in-vitro research | Nerve Repair, Hemostats, Sealants, Coils, Sponges, Spheres/Beads, Ligaments, Strings, Tubes/Cylinders, Films

Note: Lyophlilized form is not recommended for formation of a 3D gel

Product Details
Source Human Neo-Natal Fibroblast Cells
Gelation Time <90 mins
Fibril Formation >0.5 abs. units
Purity >99.9%
pH approx 2
Telo vs. Atelo Atelo-collagen
Physical Appearance Sterile filtered white lyophilized (freeze-dried) powder
Endotoxin Level ≤1.00 EU/ml as measured by kinetic LAL
Sterilization Method Filtration
Sterility No growth
Cell Attachment Assay Pass
Reconstitution Dilute in 5 ml 0.01N HCl to yield 3 mg/ml solution
SDS-PAGE

≥ 85% collagen contained within α, β, and γ bands; ≤ 15% collagen contained with bands traveling faster than α

Storage and Stability

Lyophilized product is very stable at -20°C (recommended 24 months).

Reconstituted material should be stored at 4°C and used within 3 months.

Protocols

Coating Procedure

1. Remove required quantity of collagen from the bottle and dispense into a dilution vessel. 2. Dilute OptiCol™ collagen in water to ~50 to 100 μg/ml (~1:30). A 0.01 N HCl solution may also be used. 3. Swirl contents gently until material is completely mixed. 4. Add appropriate amount of diluted OptiCol™ collagen material to the culture surface ensuring that the entire surface is coated. 5. Incubate at room temperature or 37°C, covered, for 1 - 2 hours. 6. After incubation, aspirate any remaining material. 7. Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces. 8. Coated surfaces are ready for use. They may also be stored at 2 - 10°C damp or air dried if sterility is maintained.